A kinetic model describing cell growth and production of highly active, recombinant ice nucleation protein in Escherichia Coli

 
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A kinetic model describing cell growth and production of highly active, recombinant ice nucleation protein in Escherichia Coli

Kyriakidis, Dimitrios
Zoumpoylis, Anastasios
Palaiomylitou, M.A.
Matis, K.A.

A structured kinetic model, which describes the production of the recombinant ice nucleation protein in different conditions, was applied. The model parameters were estimated based on the variation of the specific growth rate and the intracellular product concentration during cultivation. The equations employed relate the cellular plasmid content or plasmid copy number with the cloned-gene expression; these correlations were successfully tested on the experimental data. The optimal nutrient conditions for the growth of Escherichia coli expressing the inaZ gene of Pseudomonas syringae were determined for the production of active ice nucleation protein. The kinetics of the cultures expressing the inaZ gene were studied in a bioreactor at different growth temperatures and nutrient conditions

Article / Άρθρο
info:eu-repo/semantics/article

inaZ gene
Plasmid copy number
Structured kinetic model
E. coli
Ice nucleation protein

Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης (EL)
Aristotle University of Thessaloniki (EN)

Αγγλική γλώσσα

2002
2009-06-29T17:00:18Z


Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης, Σχολή Θετικών Επιστημών, Τμήμα Χημείας

urn:ISSN:00063592
Biotechnology and Bioengineering, vol.78 no.3 [2002] p.321-332 [Published Version]

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info:eu-repo/semantics/openAccess



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