assessment titanium dioxide nanoparticle as treatment of Aeromonas hydrophila infection in Oreochromis niloticus
animal health research institute
Nanoproducts became widely used materials all over the world. Antimicrobial properties of titanium dioxide (TiO2) nanoparticle (NP) were examined against Aeromonas hydrophila (A. hydrophila) bacteria and the minimum inhibitory concentration MIC was found to be 20 µg.l-1 of TiO2 NP. Also, the efficacy of TiO2 NP was examined in Oreochromis niloticus (O. niloticus) infected with A. hydrophila.One hundred and eighty of O. niloticus (54±2.4 g b.w.) were divided into six groups (G). O. niloticus in G1, G2 and G3 were fed with 0, 20 and 100 µg/g b.w. respectively while G4, G5 and G6 were i.p. injected with 0.2 ml distal water and, 20 and 100 µg.g b.w. TiO2 NP respectively for three times with ten days of interval.The blood parameters and some of biochemical parameters of O. niloticus, that received high dosage of TiO2 NP were significantly impacted regardless the administration rout. Elevation of the activities of glutathione peroxidase (GPx) and metallothionine (MT) were recorded with high dosage. O. niloticus subjected to high dosage of TiO2 NP had the lower survival rate SR% especially with injection (50%). The mortality rate (MR)% of challenged O. niloticus against A. hydrophila was decreased with lower dosage, as G2 and G5 20 and 30% respectively. So, 20 µg.g-1 of TiO2 NP could be safely protect O. niloticus against A. hydrophila infection since no health hazards was observed. While, health status of O. niloticus was adversely impacted with high dosage of TiO2 NP regardless the rout of administration.