Neopterine and Soluble Urokinase Type Plasminogen Activator Receptor as Novel Biomarkers in Dogs with Systemic Inflammatory Response Syndrome
The Coordinator of Scientific Research Projects of Sivas Cumhuriyet University
There is a high mortality rate in dogs with systemic inflammatory response syndrome (SIRS). This study aims to investigate the diagnostic value of markers C-reactive protein (CRP), soluble urokinase-type plasminogen activator receptor (suPAR) and neopterine in dogs with SIRS. The materials of the study consist of 30 dogs with SIRS and 15 healthy dogs that show no disease symptom, 45 dogs in total. CRP, suPAR and neopterin levels of the serum samples were determined in the ELISA reader by using commercial kits by the sandwich enzyme immunoassay method. In the study, it was found that CRP, suPAR and neopterine levels of the dogs with SIRS were significantly higher (P < 0.001, P < 0.007 and P < 0.007) than those of the dogs in the healthy group. The cut-off values for the healthy dogs and dogs with SIRS for CRP, suPAR and neopterine were determined to be 11.20 mg/mL, 223.68 ng/L and 3.23 nmol/mL, respectively. At these cut-off values, high levels of sensitivity (92.9% for CRP, 53.3% for suPAR and 63.3% for neopterine) and specificities (93.3%) were detected.In conclusion, SuPAR and neopterine levels can be utilized alternative to CRP measurement from serum as an indicator of acute inflammation in dogs with SIRS. It was also argued that further studies were necessary in order to determine diagnosis and prognosis of diseases.