Fetal bovine serum is associated with polar body degeneration after in vitro maturation of bovine oocytes

 
Το τεκμήριο παρέχεται από τον φορέα :

Αποθετήριο :
Journal of the Hellenic Veterinary Medical Society  | ΕΚΤ eJournals
δείτε την πρωτότυπη σελίδα τεκμηρίου
στον ιστότοπο του αποθετηρίου του φορέα για περισσότερες πληροφορίες και για να δείτε όλα τα ψηφιακά αρχεία του τεκμηρίου*
κοινοποιήστε το τεκμήριο




2018 (EL)

Fetal bovine serum is associated with polar body degeneration after in vitro maturation of bovine oocytes (EL)
Fetal bovine serum is associated with polar body degeneration after in vitro maturation of bovine oocytes (EN)

MACEDO, S.
GONZÁLEZ-FERNÁNDEZ, L.
MACÍAS-GARCÍA, B.
ROCHA, A.

In vitro fertilization (IVF) in cattle is commonly used worldwide. Although extensive research has been conducted using different additives in the different IVF steps, little is known regarding how protein type may affect bovine oocytes during the fertilization period. In addition, unlike Tissue Culture Medium 199 (TCM), fertilization medium may induce oocytes’ chromatin degeneration during prolonged incubation in the horse (Modified Whitten’s medium). Thus, in the present work TCM-199 supplemented with either 7 mg/ml of Bovine Serum Albumin (TCM+BSA) or 10% Fetal Bovine Serum (v/v; TCM+FBS) was used. Bovine oocytes were matured in vitro and placed in the previously mentioned media for further 18 hours, in the absence of added sperm (sham fertilization) and their chromatin conformation was evaluated. After IVM, 78.9% of the initial oocytes had reached the MII stage. After sham fertilization, 58.6% of the oocytes in TCM+BSA while just 28.3% in TCM+FBS maintained the MII chromatin conformation (p < 0.05). Subsequent experiments run using PB extruded oocytes and incubated in TCM+BSA and TCM+FBS during sham fertilization, demonstrated that FBS was consistently associated with polar body dissolution or degeneration. (EN)

info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion

BSA (EL)
oocyte (EL)
polar body (EL)
FBS (EL)
bovine (EL)
BSA (EN)
oocyte (EN)
polar body (EN)
FBS (EN)
bovine (EN)


Journal of the Hellenic Veterinary Medical Society

Αγγλική γλώσσα

2018-01-29


Hellenic Veterinary Medical Society / Ελληνική Κτηνιατρική Εταιρεία (EN)

1792-2720
2585-3724
Περιοδικό της Ελληνικής Κτηνιατρικής Εταιρείας; Τόμ. 68 Αρ. 3 (2017); 279-284 (EL)
Journal of the Hellenic Veterinary Medical Society; Vol. 68 No. 3 (2017); 279-284 (EN)

Copyright (c) 2018 B MACÍAS-GARCÍA, S MACEDO, A ROCHA, L GONZÁLEZ-FERNÁNDEZ (EN)



*Η εύρυθμη και αδιάλειπτη λειτουργία των διαδικτυακών διευθύνσεων των συλλογών (ψηφιακό αρχείο, καρτέλα τεκμηρίου στο αποθετήριο) είναι αποκλειστική ευθύνη των αντίστοιχων Φορέων περιεχομένου.