Detection of Mycobacterium avium subsp. paratuberculosis by several diagnostics techniques in clinical suspected sheep

 
see the original item page
in the repository's web site and access all digital files if the item*
share




2018 (EN)
Ανίχνευση του Mycobacterium avium subsp. paratuberculosis με διάφορες διαγνωστικές τεχνικές σε κλινικά ύποπτα πρόβατα (EL)
Detection of Mycobacterium avium subsp. paratuberculosis by several diagnostics techniques in clinical suspected sheep (EN)

COELHO, A. C.
PINTO, M. L.
PIRES, M. A.
COELHO, A. M.
GARCÍA-DIEZ, J.

ΔΕΝ ΥΠΑΡΧΕΙ ΕΛΛΗΝΙΚΗ ΠΕΡΙΛΗΨΗ (EL)
A total of thirty sheep with clinical symptomatology of paratuberculosis (Johne’s disease) were subjected to four diagnostic techniques: histopathological examination, bacteriological culture (in faeces and tissues), polymerase chain reaction (PCR) (in blood, tissue and faecal samples) and antibody responses (ELISA). Twenty-one (70.0%) animals showed histological lesions. Bacterial culture of both faeces and tissue revealed that 2 (6.7%) and 6 (20.0%) of the 30 sheep were infected, respectively. Mycobacterium avium subsp. 24 paratuberculosis (Map) was identified in 4 animals via PCR of faeces (13.3%), and in 19 (63.3%) by PCR in tissues. PCR in blood revealed 7 (23.3%) infected animals. Three (10.0%) animals showed antibodies against Map. A greater number of positive animals were detected by histopathological examination and PCR in tissues than by culture or ELISA. This study confirmed the clinical findings and results suggest that histopathology, PCR in tissues and in blood can improve the detection of Map in physically suspected animals and should be considered useful tools in the diagnosis of Map in suspected sheep. (EN)

info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion

Πρόβατο (EL)
ιστοπαθολογία (EL)
παραφυματίωση (EL)
PCR (EL)
καλλιέργεια (EL)
ELISA (EL)
paratuberculosis (EN)
culture (EN)
histopathology (EN)
sheep (EN)
PCR (EN)
ELISA (EN)

Ελληνική Κτηνιατρική Εταιρεία (EL)
Hellenic Veterinary Medical Society (EN)

2018-01-29


Hellenic Veterinary Medical Society / Ελληνική Κτηνιατρική Εταιρεία (EN)

1792-2720
2585-3724
Journal of the Hellenic Veterinary Medical Society; Vol 68, No 2 (2017); 167-174 (EL)
Journal of the Hellenic Veterinary Medical Society; Vol 68, No 2 (2017); 167-174 (EN)

Copyright (c) 2018 AC COELHO, AM COELHO, J GARCÍA-DIEZ, MA PIRES, ML PINTO (EN)



*Institutions are responsible for keeping their URLs functional (digital file, item page in repository site)