Effect of genistein addition to equine sperm freezing extender

 
Το τεκμήριο παρέχεται από τον φορέα :

Αποθετήριο :
Journal of the Hellenic Veterinary Medical Society  | ΕΚΤ eJournals
δείτε την πρωτότυπη σελίδα τεκμηρίου
στον ιστότοπο του αποθετηρίου του φορέα για περισσότερες πληροφορίες και για να δείτε όλα τα ψηφιακά αρχεία του τεκμηρίου*
κοινοποιήστε το τεκμήριο



Σημασιολογικός εμπλουτισμός από το EKT
Τμήμα περιοδικού (EL)
Επιστημονικό άρθρο (EL)
2018 (EL)
Effect of genistein addition to equine sperm freezing extender (EL)
Effect of genistein addition to equine sperm freezing extender (EN)
LOPES, G.
GUIMARÃES, Τ.
GONZÁLEZ-FERNÁNDEZ, L.
MACÍAS-GARCÍA, Β.
ROCHA, A.
ΔΕΝ ΥΠΑΡΧΕΙ ΠΕΡΙΛΗΨΗ (EL)
Cryopreservation negatively affects equine sperm quality post-thaw: an excessive release of reactive oxygen species (ROS) and increased protein tyrosine phosphorylation (known as cryocapacitation) have been demonstrated in frozenthawed equine sperm diminishing its lifespan. The objective of this study was to determine the possible beneficial effect of genistein addition (a ROS scavenger and protein tyrosine kinase inhibitor) to a commercial freezing extender. Equine sperm were frozen in the presence of different genistein concentrations ranging from 0 to 800 μM. After thawing, the sperm viability (eosinnigrosin), acrosome integrity using peanut agglutinin (PNA), protein tyrosine phosphorylation (PY) and motility were assessed at times 0 and 1 hr. In addition PY was studied in fresh and frozen sperm incubated in Modified Whitten’s (MW) medium with 25 mM Bicarbonate (MW+Bic). Genistein did not affect the viability, total or progressive motility or acrosome status of frozenthawed equine sperm. Immediately after thawing, positive PY staining in the equatorial band was observed and genistein addition did not exert any change in PY. Fresh sperm incubated in MW+Bic showed a significant increase in PY staining along the tail compared to frozen-thawed sperm. In our study sperm viability immediately post-thaw was 58.25% ± 5.35 and progressive motility 14.46% ± 5.7 (mean ± S.E.M.) and genistein did not improve the post-thaw quality of equine sperm. In addition, cryopreservation did not induce PY immediately post-thaw or enhanced frozen-thawed equine sperm susceptibility to PY induction. (EN)
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
antioxidants (EL)
sperm (EL)
cryopreservation (EL)
equine (EL)
antioxidants (EN)
sperm (EN)
cryopreservation (EN)
equine (EN)
Ελληνική Κτηνιατρική Εταιρεία
Journal of the Hellenic Veterinary Medical Society
Research Articles
Journal of the Hellenic Veterinary Medical Society
Αγγλική γλώσσα
2018-01-31
https://ejournals.epublishing.ekt.gr/index.php/jhvms/article/view/15868
Hellenic Veterinary Medical Society / Ελληνική Κτηνιατρική Εταιρεία (EN)
1792-2720
2585-3724
Περιοδικό της Ελληνικής Κτηνιατρικής Εταιρείας; Τόμ. 66 Αρ. 4 (2015); 241-248 (EL)
Journal of the Hellenic Veterinary Medical Society; Vol. 66 No. 4 (2015); 241-248 (EN)
https://creativecommons.org/licenses/by-nc/4.0 (EN)
Copyright (c) 2018 Β MACÍAS-GARCÍA, Τ GUIMARÃES, G LOPES, A ROCHA, L GONZÁLEZ-FERNÁNDEZ (EN)



*Η εύρυθμη και αδιάλειπτη λειτουργία των διαδικτυακών διευθύνσεων των συλλογών (ψηφιακό αρχείο, καρτέλα τεκμηρίου στο αποθετήριο) είναι αποκλειστική ευθύνη των αντίστοιχων Φορέων περιεχομένου.