Το γονίδιο Cux2 στην ανάπτυξη των ενδιάμεσων νευρώνων του φλοιού

 
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2014 (EN)
Cux2 in cortical interneuron development
Το γονίδιο Cux2 στην ανάπτυξη των ενδιάμεσων νευρώνων του φλοιού

Kessaris, Nikoletta
Βελώνα, Θεοδώρα

Πανεπιστήμιο Κρήτης.

The Cux2 gene encodes a homeodomain transcription factor which is strongly expressed in the vertebrate nervous system during development. It is one of the two orthologue genes of the Drosophila cut gene which patterns a specific sensory organ in the peripheral nervous system and has been implicated in the regulation of dendritic branching in the central nervous system. During mouse development Cux2 is expressed in a group of progenitors in the subventricular zone (SVZ) of the dorsal telencephalon and in interneurons migrating from the ganglionic eminences into the pallium. In postnatal and adult animals CUX2 is localized mainly in the upper layers and sparsely in the lower layers of the cortex. Previous studies have shown that CUX2, together with CUX1 regulate the dendritic branching, spine development and synaptic formation in layers II-III of the cortex. Also, CUX2 regulates the proliferation rates in the cortical SVZ and the numbers of neurons in the upper layers of the cortex. Until now, the contribution of CUX2 in the formation of different GABAergic interneuron populations in the cortex has been poorly assessed. In this project we investigated the possible roles of CUX2 in the development of cortical interneurons. We used a conditional knock-out mouse model (Nkx2.1-Cre; Cux2 fl/Δ) in which CUX2 was deleted in interneurons derived from the medial ganglionic eminence (MGE), one of the two major sources of interneurons for the cortex. Our results, deriving from immunohistochemistry and in situ hybridization analysis, suggest 1) an increase in PV-expressing interneuron numbers and 2) an increase in soma- and axon initial segment-targeting inhibitory synapses in the cortex. These results are supported by electrophysiological data showing raised inhibition, as a functional consequence of CUX2 deletion in cortical circuits. Finally, behavioural assessment revealed defects in sociability. (EN)

text
Τύπος Εργασίας--Μεταπτυχιακές εργασίες ειδίκευσης

Πανεπιστήμιο Κρήτης (EL)
University of Crete (EN)

English

2014-12-04


Σχολή/Τμήμα--Ιατρική Σχολή--Τμήμα Ιατρικής--Μεταπτυχιακές εργασίες ειδίκευσης



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