Wang, YiLiang, GuangAnsari, DanielSaleem, Moin A.Tang, LongguangRosendahl, Ann H.Andersso, RolandBackground Pancreatic cancer is a devastating disease with poor prognosis. It is characterized by a pronounced stromal reaction, which resists chemotherapeutics and effective tumor treatment. Pancreatic stellate cells (PSCs) are mainly responsible for this stromal reaction. Moreover, the cancer and stromal interaction seems to promote tumor proliferation. In this study, L49H37, a newly synthesized curcumin analog, was used as intervention to target the stromal compartment of pancreatic cancer.Methods In vitro cultures of human PSCs were exposed to curcumin and L49H37. Cell viability as well as growth promoting and survival signaling pathways were monitored by MTT, flow cytometry and western blotting.Results Curcumin and L49H37 effectively inhibited proliferation and induced apoptosis in PSCs. L49H37 was found to be more potent at a lower concentration than curcumin in the induction of apoptosis, as evidenced by cleaved poly (ADP-ribose) polymerase (PARP). The cells were retained in the G0/G1 phase of the cell cycle through the downregulation of p21WAF1/Cip1. L49H37 significantly decreased the phosphorylation of extracellular signal regulated kinase ½ (ERK½).Conclusion The results indicate that curcumin analog L49H37 exhibits more potent inhibitory effects than curcumin itself at a lower concentration, which suggests that it may have a potential for further evaluation of its use against pancreatic adenocarcinoma, either as a single agent but, more probable, as part of a combination regimen.Keywords Pancreatic cancer, stroma, cleaved PARP, p21WAF1/Cip1Ann Gastroenterol 2015; 28 (3): 389-396text/htmlapplication/pdfapplication/mswordapplication/vnd.openxmlformats-officedocument.wordprocessingml.documenthttp://www.annalsgastro.gr/index.php/annalsgastro/article/view/2094engHellenic Society of Gastroenterology1792-74631108-7471Annals of Gastroenterology; Volume 28, No 3 (2015); 391Antiproliferative effects of curcumin analog L49H37 in pancreatic stellate cells: a comparative studyinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion2015-06-11Δημοσίευση σε περιοδικόPublication in journalΓαστροεντερολογίαGastroenterologyisi -Gastroenterology & Hepatology covers resources on the anatomy, physiology, biochemistry, and pathology of the digestive system. This category includes specific resources on the prognosis and treatment of digestive diseases; stomach ulcers; metabolic, genetic, infectious and chemically induced diseases of the liver; colitis; diseases of the pancreas and diseases of the rectum.Επιστημονικό άρθροScientific articleΕλληνική Γαστροεντερολογική ΕταιρίαGreek Aggregator OpenArchives.gr | National Documentation Centre (EKT)other