Portal sinusoids' neutrophil plugging after experimental ischemia reperfusion liver injury

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2007 (EN)
Portal sinusoids' neutrophil plugging after experimental ischemia reperfusion liver injury (EN)

D. Paramythiotis, Å. Eleftheriadis, K. Kotzampassi, Ch. Kalekou,

ABSTRACT The aim of the present study was the demonstration of a relationship between reduced liver microcirculation - after different periods of ischemia-reperfusion - and the number of neutrophils plugged to the microvasculature at reperfusion. Ninety male Wistar rats were included in the study. These were subdivided into control group, 30min ischemia and 60min ischemia groups as well as 30min ischemia - 60min reperfusion and 60min ischemia - 60min reperfusion groups. Samples of liver tissue were obtained for the assessment of the number of neutrophils entrapped within portal sinusoids, after histochemical staining and morphometry. Hepatic microcirculation was estimated by laser-Doppler flowmetry. Additionally, ALT serum levels were evaluated, as an established marker of liver ischemiareperfusion injury. A course of 60min ischemia - 60min reperfusion resulted to a statistically significant increase of neutrophils plugged within liver sinusoids in relation to the 30min ischemia - 60min reperfusion [p=0.001]. Similarly, hepatic tissue microcirculation exhibited a flux recovery of 70% in 30min ischemia - 60min reperfusion group and only 57% in the 60min ischemia - 60min reperfusion group [p=0,001]. Serum ALT activities were found significantly increased during reperfusion, in both groups. It is thus concluded that capillary perfusion failure occurring during liver reperfusion depends on ischemia time and is associated with a significant increase of neutrophils’ accumulation into liver sinusoids. Key words: liver, ischemia/reperfusion injury, neutrophils, microcirculation (EN)




Hellenic Society of Gastroenterology (EN)

Annals of Gastroenterology; Volume 16, No 4 (2003) (EN)

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