Naloxone: a potent protective agent in ischemia – reperfusion – induced liver injury

 
This item is provided by the institution :

Repository :
Annals of Gastroenterology
see the original item page
in the repository's web site and access all digital files if the item*
share




2007 (EN)
Naloxone: a potent protective agent in ischemia – reperfusion – induced liver injury (EN)

A. Kolettas, Georgia Lazaraki, E. Eleftheriadis, Katerina Kotzampassi, D. Paramythiotis,

Hepatic ischemia/reperfusion-induced injury is accompanied by free radical production, leading to endothelial cell destruction, adhesion of neutrophils and plugging of the hepatic sinusoids and thus to blood microcirculation flow reduction. Naloxone is a known opioid antagonist that has been shown to act by inhibiting the release of free radicals. The purpose of this study is to clarify the changes in hepatic microcirculation during liver ischemia and after reperfusion in the rat and the potential beneficial effects of Naloxone as pretreatment, in respect to microcirculation and oxidative stress. One hundred and forty male Wistar rats, allocated to Naloxone [N] or Placebo [P] treatment, were subjected to either 30min or 60min normothermic ischemia [70% of total liver], followed by 60min reperfusion. Liver microcirculation was assessed by laser-Doppler flowmetry at baseline, at the end of the ischemia period [30min or 60min, respectively] and at the end of the reperfusion period, while oxidative stress was assessed by means of MDA at the same time periods. Naloxone pretreatment seemed to protect liver parenchyma, since MDA levels were significantly decreased in relation to placebo treated rats, in both 30min ischemia/ 60min reperfusion and 60min ischemia/60min reperfusion groups. Similarly, Naloxone pretreatment was found to significantly improve liver microcirculation in relation to placebo treated rats, in both groups. In conclusion, the results of this study indicate that Naloxone pretreatment protects the liver from ischemia/ reperfusion hepatocellular injury. Key words: Naloxone, ischemia/reperfusion injury, liver, laser- Doppler flowmetry, malondialdehyde (EN)

info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion

Ελληνική Γαστροεντερολογική Εταιρία (EL)
Hellenic Society of Gastroenterology (EN)

2007-03-19


Hellenic Society of Gastroenterology (EN)

1792-7463
1108-7471
Annals of Gastroenterology; Volume 18, No 4 (2005) (EN)



*Institutions are responsible for keeping their URLs functional (digital file, item page in repository site)