The impact of corticosteroids on hepatocytes ultrastructure experimental study in rats with electron microscopy
Background and Aim: Corticosterstoids are drugs widely
used in common clinical practice.Their effect on liver morphology
and function is not fully elucidated.The aim of this
expenmental study is to investigate the impact of cortisol
on fasted rat hepatocytes, by means of electron microscopy.
Materials and Methods: Fifty male Wistar rats weighing
130-150gr were divided into two experimental groups: Group
I (N=30): Hydrocortisol 8mg/100gr i.p. and Group II (N-
20): Normal saline i.p. Animals were sacrificed 30 min and
180 min after injection and liver specimens were taken. According
to OsO4 -fixation protocol, ultra fine tissue sections
were obtained and stained for electron microscopy.
Results: The following findings were observed in >90% of
cortisol treated animals and were significantly more frequent
than in control group (P