Surgical guide including innovative techniques for orthotopic liver transplantation in the rat: Key techniques and pitfalls in whole and split liver grafts
δείτε την πρωτότυπη σελίδα τεκμηρίου στον ιστότοπο του αποθετηρίου του φορέα για περισσότερες πληροφορίες και για να δείτε όλα τα ψηφιακά αρχεία του τεκμηρίου*
Surgical guide including innovative techniques for orthotopic liver transplantation in the rat: Key techniques and pitfalls in whole and split liver grafts
(EN)
Hori, T.
Uemoto, S.
Nguyen, J.H.
Ohashi, N.
Kamo, N.
Castanedes-Casey, M.
Zhao, X.
Conkle, F.
Rousseau, L.G.
Chen, F.
Baine, A-M T.
Murray, M.
Phillips, V.R.
Gardner, L.B.
Background. Although the techniques of orthotopic liver transplantation (OLT) and split OLT (S-OLT) have been well documented in the rat by established microsurgeons, the surgical skills required for this model limits its use by some investigators. Furthermore, alternative artificial OLT/S-OLT models are of limited use in the liver transplantation field. Herein, we describe detailed surgical procedures for our rat OLT/S-OLT model and our surgical learning curves. Methods. We studied the anatomical findings, including inflow and outflow, in 100 rats following OLT/S-OLT, and determined the operator learning curves for this model. We also investigated the discrepancy between survival rates and the rates of reliable samples in OLT and S-OLT, respectively, because surgical issues destroy all experiments. Results. Learning curve data indicated that 50 cases were required for operator training plus sufficient animals to start a study. In making split-liver grafts, thoughtful consideration of inflow and outflow are crucial for successful S-OLT. Our results showed that some complications necessarily disrupt all of the experiments, and we should omit unreliable samples if any complications and/or unreasonable histology are observed, especially at early post-operative period after S-OLT. Conclusion. Although OLT/S-OLT in rats required advanced skills, this is the only liver transplantation model that provides clinically relevant and reliable results.
(EN)
Annals of Gastroenterology; Volume 23, No 4 (2010); 270 - 295
(EN)
*Η εύρυθμη και αδιάλειπτη λειτουργία των διαδικτυακών διευθύνσεων των συλλογών (ψηφιακό αρχείο, καρτέλα τεκμηρίου στο αποθετήριο) είναι αποκλειστική ευθύνη των αντίστοιχων Φορέων περιεχομένου.
Βοηθείστε μας να κάνουμε καλύτερο το OpenArchives.gr.