Surgical guide including innovative techniques for orthotopic liver transplantation in the rat: Key techniques and pitfalls in whole and split liver grafts

 
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Annals of Gastroenterology
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Surgical guide including innovative techniques for orthotopic liver transplantation in the rat: Key techniques and pitfalls in whole and split liver grafts (EN)

Hori, T.
Uemoto, S.
Nguyen, J.H.
Ohashi, N.
Kamo, N.
Castanedes-Casey, M.
Zhao, X.
Conkle, F.
Rousseau, L.G.
Chen, F.
Baine, A-M T.
Murray, M.
Phillips, V.R.
Gardner, L.B.

Background. Although the techniques of orthotopic liver transplantation (OLT) and split OLT (S-OLT) have been well documented in the rat by established microsurgeons, the surgical skills required for this model limits its use by some investigators. Furthermore, alternative artificial OLT/S-OLT models are of limited use in the liver transplantation field. Herein, we describe detailed surgical procedures for our rat OLT/S-OLT model and our surgical learning curves. Methods. We studied the anatomical findings, including inflow and outflow, in 100 rats following OLT/S-OLT, and determined the operator learning curves for this model. We also investigated the discrepancy between survival rates and the rates of reliable samples in OLT and S-OLT, respectively, because surgical issues destroy all experiments. Results. Learning curve data indicated that 50 cases were required for operator training plus sufficient animals to start a study. In making split-liver grafts, thoughtful consideration of inflow and outflow are crucial for successful S-OLT. Our results showed that some complications necessarily disrupt all of the experiments, and we should omit unreliable samples if any complications and/or unreasonable histology are observed, especially at early post-operative period after S-OLT. Conclusion. Although OLT/S-OLT in rats required advanced skills, this is the only liver transplantation model that provides clinically relevant and reliable results. (EN)

info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion

Ελληνική Γαστροεντερολογική Εταιρία (EL)
Hellenic Society of Gastroenterology (EN)

2010-10-14


Hellenic Society of Gastroenterology (EN)

1792-7463
1108-7471
Annals of Gastroenterology; Volume 23, No 4 (2010); 270 - 295 (EN)



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