Biological variations in a long-term ex situ cultivation: a Mediterranean demosponge as model system

 
This item is provided by the institution :

Repository :
Mediterranean Marine Science  | ΕΚΤ eJournals
see the original item page
in the repository's web site and access all digital files if the item*
share




2014 (EN)
Biological variations in a long-term ex situ cultivation: a Mediterranean demosponge as model system (EN)

CARDONE, F.
CORRIERO, G.
SCOCCIA, F.
GAINO, E.
LIUZZI, G. M.
NONNIS MARZANO, C.
DI BARI, G.

Italian Ministry of Education, University and Research (EN)

The demosponge Tethya citrina Sarà & Melone was subjected to long-term aquarium farming, considering that several species belonging to this genus are a rich source of useful bioactive metabolites. The rearing method in the present research was mainly based on applying parameters taken from the current literature. The sponge's biological activity was assessed in terms of survival and growth over time; status of filtering apparatus; protein banding profiles. Farmed sponges showed a high survival rate and marked reduction in size. In addition, they were frequently affected by massive production of asexual buds. Histological and ultrastructural observations showed a gradual disorganization of the sponge choanocyte chambers, with a drastic reduction in choanocytes and a progressive increase in spherulous cells. Comparative electrophoretic analysis of the protein profiles of wild and reared specimens showed differences in protein composition and abundance between the tested groups. Such data are consistent with the increase in spherulous cells whose content was markedly electron-dense, a feature stressing the proteinaceous nature of these inclusions. Experimental tank-rearing of Tethya provided promising responses in terms of high survival rate of the species and easy reproduction, though the rearing protocol used led to profound morpho-functional changes in the sponge. This confirms the inadequate state of knowledge on farming techniques for this taxon, while also highlighting potential applications in biotechnology of ex situ breeding techniques, in order to modify the sponges' biological responses. (EN)

info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion

ex situ rearing (EN)
Tethya citrina (EN)
tissue reorganization (EN)
protein profile (EN)
bioactive compound (EN)

Ελληνικό Κέντρο Θαλασσίων Ερευνών (ΕΛΚΕΘΕ) (EL)
Hellenic Centre for Marine Research (HCMR) (EN)

Mediterranean Marine Science

2014-09-28


Hellenic Centre for Marine Research (EN)

marine sponges (EN)
2010-11 (EN)
Mediterranean Sea (EN)

1791-6763
1108-393X
Mediterranean Marine Science; Vol 16, No 1 (2015); 73-81 (EL)
Mediterranean Marine Science; Vol 16, No 1 (2015); 73-81 (EN)



*Institutions are responsible for keeping their URLs functional (digital file, item page in repository site)