p53 expression and mutations in squamous cell carcinoma of the head and neck: expression correlates with the patients' use of tocacco and alcohol

 
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p53 expression and mutations in squamous cell carcinoma of the head and neck: expression correlates with the patients' use of tocacco and alcohol

Zoumpourlis, Vassilis
Spandidos, D. A.
Jones, Andrew S.

P53 expression was assessed in 93 head and neck squamous cell carcinoma patients using CM-1, DO-7, and DO-1 antibodies. Sixty eight percent was found to have positive nuclear staining. The frequency of p53 mutations were investigated in 13 patients using single-stranded conformational polymorphism (SSCP) analysis of DNA fragments that had been amplified by the polymerase chain reaction (PCR). P53 gene mutations were analyzed by SSCP in exons 4 to 7 of 13 patients, and nine were found to have mutations in exon 4; two of these patients also had a mutation in exon 5. In the group of 93 patients, p53 overexpression was found to correlate with the patients' history of heavy smoking (p <0.01). We also analyzed the drinking and smoking history of 71 of these patients by logistic regression analysis and found that heavy smoking correlates with p53 overexpression (p <0.05), but heavy drinking was not found to be significant. However when both smoking and drinking histories were assessed together, a correlation was found (p <0.05). Future work may indicate that specific p53 mutations are associated with patients who have a history of heavy drinking and smoking.

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Αγγλική γλώσσα

2008-10-02T09:23:37Z
1994
2012-06-12T12:10:17Z
2008-12-10T13:55:29Z


Τύποι Τεκμηρίων
Άρθρο σε περιοδικό
Συλλογές του Ήλιος
Ινστιτούτο Βιολογικών Ερευνών και Βιοτεχνολογίας (ΙΒΕΒ) (έως 2012)
Ινστιτούτο Bιολογίας, Φαρμακευτικής Χημείας και Βιοτεχνολογίας (ΙΒΦΧΒ)



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