This item is provided by the institution :
Technological Educational Institute of Athens
Repository :
Ypatia - Institutional Repository
see the original item page
in the repository's web site and access all digital files if the item*

2014 (EN)
Short communication (EN)

Σταματόπουλος, Κωνσταντίνος (EL)
Χατζηλαζάρου, Αρχοντούλα (EL)
Τσάκαλη, Ευσταθία (EL)
Πετρωτός, Κωνσταντίνος Β. (EL)
D'Alessandro, Angela Gabriella (EN)

Γούλας, Παναγιώτης (EL)
Μασούρας, Θεόφιλος (EL)
Τεχνολογικό Εκπαιδευτικό Ίδρυμα Αθήνας. Σχολή Τεχνολογίας Τροφίμων και Διατροφής. Τμήμα Οινολογίας και Τεχνολογίας Ποτών (EL)
Van Impe, Jan Frans M. (EN)

In the current paper, a method is introduced to determine lactoferrin in sweet whey using reversed-phase HPLC without any pretreatment of the samples or use of a separation technique. As a starting point, the most common HPLC protocols for acid whey, which included pretreatment of the whey along with a sodium dodecyl sulfate-PAGE step, were tested. By skipping the pretreatment and the separation steps while altering the gradient profile, different chromatographs were obtained that proved to be equally efficient to determine lactoferrin. For this novel 1-step reversed-phase HPLC method, repeatability was very high over a wide range of concentrations (1.88% intraday to 5.89% interday). The limit of detection was 35.46 μg/mL [signal:noise ratio (S/N) = 3], whereas the limit of quantification was 50.86 μg/mL (S/N = 10). Omitting the pretreatment step caused a degradation of the column’s lifetime (to approximately 2,000 samples). As a result, the lactoferrin elution time changed, but neither the accuracy nor the separation ability of the method was significantly influenced. We observed that this degradation could be easily avoided or detained by centrifuging the samples to remove fat or by extensive cleaning of the column after every 5 samples. (EN)


Lactoferrin (EN)
Φέτα (EN)
Υγρή χρωματογραφία υψηλής απόδοσης αντίστροφης φάσης (EN)
reversed-phase high performance liquid chromatography (EN)
Whey (EN)
Feta cheese (EN)
Τυρόγαλο (EN)

ΤΕΙ Αθήνας (EL)
Technological Educational Institute of Athens (EN)

N/A (EN)




American Dairy Science Association (EN)

*Institutions are responsible for keeping their URLs functional (digital file, item page in repository site)