On the mechanism of direct conversion of cellulose to ethanol by Fusarium oxysporum: Effect of cellulase and β-glucosidase
(EN)
Christakopoulos, P
(EN)
Macris, BJ
(EN)
Kekos, D
(EN)
The effects of the three main enzymes involved in cellulose saccharification, namely cellobiohydrolase, carboxymethylcellulase and β-glucosidase, on the direct conversion of cellulose to ethanol by Fusarium oxysporum F3 were investigated. Ethanol production was not affected when the activity of the former two enzymes was varied within a wide range. By contrast, β-glucosidase markedly affected ethanol production showing an optimum level of 0.7-0.8 unit/ml growth medium. A significant decrease of cellulose bioconversion time to ethanol was obtained when β-glucosidase activity was adjusted to this optimal level at the beginning of the fermentation process.
(EN)
