OPTIMIZATION OF BETA-GLUCOSIDASE CATALYZED SYNTHESIS OF TRISACCHARIDES FROM CELLOBIOSE AND GENTIOBIOSE

 
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1994 (EN)

OPTIMIZATION OF BETA-GLUCOSIDASE CATALYZED SYNTHESIS OF TRISACCHARIDES FROM CELLOBIOSE AND GENTIOBIOSE (EN)

CHRISTAKOPOULOS, P (EN)
GOODENOUGH, PW (EN)
MACRIS, BJ (EN)
BHAT, MK (EN)
KEKOS, D (EN)

Purified beta-Glucosidase from Fusarium oxysporum catalysed the hydrolysis and transglycosylation reactions in the presence of cellobiose and gentiobiose. The product of the latter reaction was mainly a triose. The time of incubation, pH and substrate concentration for transglycosylation reaction were optimised. Under optimal conditions, the concentration of glucose and triose reached approximately 15-20 % of the initial substrate concentration. These results suggested that beta-glucosidase from F.oxysporum is an ideal enzyme for the synthesis of triose in reasonable quantities. (EN)

journalArticle (EN)

PURIFICATION (EN)
OLIGOSACCHARIDE SYNTHESIS (EN)
Biotechnology & Applied Microbiology (EN)


BIOTECHNOLOGY LETTERS (EN)

English

1994 (EN)

ISI:A1994NW11900008 (EN)
587 (EN)
0141-5492 (EN)
16 (EN)
6 (EN)
10.1007/BF00128605 (EN)
592 (EN)

CHAPMAN HALL LTD (EN)




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