Cross-synergism in enzymatic hydrolysis of lignocellulosics: Mathematical correlations according to a hyperbolic model

 
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Cross-synergism in enzymatic hydrolysis of lignocellulosics: Mathematical correlations according to a hyperbolic model (EN)

Christakopoulos, P (EN)
Tarantili, PA (EN)
Koukios, EG (EN)
Macris, BJ (EN)
Koullas, DP (EN)
Kekos, D (EN)

N/A (EN)

The effect of cross-synergism in enzymatic hydrolysis of ball-milled Avicell, alkali-treated straw cellulose (ATSC), cotton and filter paper was investigated using mixtures of Fusarium oxysporum and Neurospora crassa enzymes. The experimental data were fitted according to an empirical hyperbolic model which utilized two parameters, the maximum conversion (x(max)) and the enzymatic hydrolysis time corresponding to 50% of x(max) (1/2). The model can predict conversion of polysaccharides as a function of hydrolysis time. Both model parameters were found to be strongly dependent on the crystallinity index as well as on the degree of delignification of the substrate. Up to 60% cellulose hydrolysis can be achieved when the crystallinity index of Avicell is reduced from 94.8% to 63.3%. The percentage increase of x(max) due to delignification was higher than the corresponding increase of t(1/2). The extent of cross-synergism depends strongly on crystallinity index and degree of delignification. This type of synergism has been found to be significant in the case of substrates which are resistant to hydrolysis, such as Avicell (with high crystallinity index) or cotton. Cross-synergistic phenomena caused by enzymatic mixtures can double cellulose hydrolysis yield with delignified straw as compared to the hydrolysis yields achieved by single-microorganism cellulases.The effect of cross-synergism in enzymatic hydrolysis of ball-milled Avicell, alkali-treated straw cellulose (ATSC), cotton and filter paper was investigated using mixtures of Fusarium oxysporum and Neurospora crassa enzymes. The experimental data were fitted according to an empirical hyperbolic model which utilized two parameters, the maximum conversion (xmax) and the enzymatic hydrolysis time corresponding to 50% of xmax (t1/2). The model can predict conversion of polysaccharides as a function of hydrolysis time. Both model parameters were found to be strongly dependent on the crystallinity index as well as on the degree of delignification of the substrate. Up to 60% cellulose hydrolysis can be achieved when the crystallinity index of Avicell is reduced from 94.8% to 63.3%. The percentage increase of xmax due to delignification was higher than the corresponding increase of t1/2. The extent of cross-synergism depends strongly on crystallinity index and degree of delignification. This type of synergism has been found to be significant in the case of substrates which are resistant to hydrolysis, such as Avicell (with high crystallinity index) or cotton. Cross-synergistic phenomena caused by enzymatic mixtures can double cellulose hydrolysis yield with delignified straw as compared to the hydrolysis yields achieved by single-microorganism cellulases. (EN)

journalArticle

Mixtures (EN)
Enzymatic hydrolysis (EN)
Maximum conversion (EN)
Cellulose (EN)
Delignification (EN)
Biomass (EN)
Straw (EN)
Avicell (EN)
Hyperbolic model (EN)
Microorganisms (EN)
Polysaccharides (EN)
Cross synergism (EN)
Energy conversion (EN)
Mathematical models (EN)
Enzymes (EN)
Fusarium oxysporum (EN)
Chemical reactions (EN)
Alkali treated straw cellulose (EN)
Cross-synergism in enzymatic hydrolysis (EN)
Filter paper (EN)
Lignocellulosics (EN)
Correlation methods (EN)
Substrates (EN)
Crystallinity index (EN)
Neurospora crassa cellulases (EN)
Hydrolysis (EN)
enzymatic hydrolysis models (EN)
pretreated lignocellulosics hydrolysis (EN)

Εθνικό Μετσόβιο Πολυτεχνείο (EL)
National Technical University of Athens (EN)

Biomass and Bioenergy (EN)

1996


Pergamon Press Inc, Tarrytown, NY, United States (EN)



*Η εύρυθμη και αδιάλειπτη λειτουργία των διαδικτυακών διευθύνσεων των συλλογών (ψηφιακό αρχείο, καρτέλα τεκμηρίου στο αποθετήριο) είναι αποκλειστική ευθύνη των αντίστοιχων Φορέων περιεχομένου.