Functional characterization of a cellulose binding xylanase from Fusarium oxysporum
(EN)
Samyn, B
(EN)
Christakopoulos, P
(EN)
Van Beeumen, J
(EN)
Nerinckx, W
(EN)
Maoris, B
(EN)
Claeyssens, M
(EN)
Kekos, D
(EN)
An extracellular endoxylanase from Fusarium oxysporum binds onto crystalline cellulose. A small peptide (similar to 2kDa) could be isolated after partial proteolysis of the native protein. It consists of 18 amino acids, is located in the C-terminal region of the protein and corresponds functionally to a cellulose binding domain (CBD), the first one to be reported in a fungal xylanase. The amino acid sequence of this peptide shows no homology with any known CBD.
(EN)
