An efficient and optimized purification procedure for the superoxide dismutase from Aspergillus niger. Partial characterization of the purified enzyme

 
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An efficient and optimized purification procedure for the superoxide dismutase from Aspergillus niger. Partial characterization of the purified enzyme (EN)

Hatzinikolaou, DG (EN)
Tsoukia, C (EN)
Macris, BJ (EN)
Kekos, D (EN)

N/A (EN)

Cu,Zn-superoxide dismutase was isolated from Aspergillus niger mycelia, harvested at the mid-logarithmic growth phase. The purification scheme aimed at the optimization of the ethanol/chloroform extraction (Tsuchihashi extraction) through response surface methodology. Upon optimum extraction conditions, it was possible to obtain electrophoretically pure enzyme preparations, by the application of one step anion exchange chromatography. The enzyme yield of this simple purification procedure was above 75% while the specific activity of the final preparation was among the highest reported for eucariotic microorganisms. The purified enzyme exhibited similar physicochemical characteristics with other Aspergillus sp. superoxide dismutases revealing an apparent tetrameric structure with a subunit molecular weight of 19 kDa, and a pl of 5.95.Cu,Zn-superoxide dismutase was isolated from Aspergillus niger mycelia, harvested at the mid-logarithmic growth phase. The purification scheme aimed at the optimization of the ethanol/chloroform extraction (Tsuchihashi extraction) through response surface methodology. Upon optimum extraction conditions, it was possible to obtain electrophoretically pure enzyme preparations, by the application of one step anion exchange chromatography. The enzyme yield of this simple purification procedure was above 75% while the specific activity of the final preparation was among the highest reported for eucariotic microorganisms. The purified enzyme exhibited similar physicochemical characteristics with other Aspergillus sp. superoxide dismutases revealing an apparent tetrameric structure with a subunit molecular weight of 19 kDa, and a pl of 5.95. (EN)

journalArticle

Biotechnology (EN)
Chromatography, Ion Exchange (EN)
Molecular Weight (EN)
Molecular structure (EN)
Superoxide dismutase (EN)
electrophoresis (EN)
Optimization (EN)
Molecular weight (EN)
Fungi (EN)
enzyme purification (EN)
Isoelectric Point (EN)
Electrophoresis (EN)
Extraction (EN)
Enzymes (EN)
Ethanol/chloroform extraction (EN)
Purification (EN)
Chromatographic analysis (EN)
Ethanol (EN)
Chloroform (EN)
Enzyme Stability (EN)
extraction (EN)
Aspergillus niger (EN)
superoxide dismutase (EN)
Protein Conformation (EN)
Anion exchange chromatography (EN)
Aspergillus sp. (EN)
Superoxide Dismutase (EN)

Εθνικό Μετσόβιο Πολυτεχνείο (EL)
National Technical University of Athens (EN)

Bioseparation (EN)

1997


Kluwer Academic Publishers, Dordrecht, Netherlands (EN)



*Η εύρυθμη και αδιάλειπτη λειτουργία των διαδικτυακών διευθύνσεων των συλλογών (ψηφιακό αρχείο, καρτέλα τεκμηρίου στο αποθετήριο) είναι αποκλειστική ευθύνη των αντίστοιχων Φορέων περιεχομένου.