Role of methanol on the catalytic behavior of β-glucosidase from Fusarium oxysporum

 
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1997 (EN)
Role of methanol on the catalytic behavior of β-glucosidase from Fusarium oxysporum (EN)

Christakopoulos, P (EN)
Tsitsimpikou, C (EN)
Macris, BJ (EN)
Makropoulou, M (EN)
Kolisis, FN (EN)
Kekos, D (EN)

N/A (EN)

Fusarium oxysporum β-glucosidase catalyses the transglycosylation and reverse hydrolysis reaction in an aqueous solution, in the presence of a second nucleophile stronger than water, such as methanol. In transglycosylation, the K(m) values of cellobiose for the trans reaction and hydrolysis were 138.2 mM and 356.5 mM respectively and the competitive role of methanol was shown. The K(m) value of methanol was calculated to be 1.41 M. In the reverse hydrolysis the influence of the donor concentration, up to 90% w/v glucose, was investigated and the competitive role of methanol as a nucleophilic acceptor of the glycosyl moiety was also demonstrated. (EN)

journalArticle

beta glucosidase (EN)
glucose (EN)
Fusarium oxysporum (EN)
fusarium oxysporum (EN)
methanol (EN)
Fusarium (EN)
catalysis (EN)
glycosylation (EN)
cellobiose (EN)
article (EN)
hydrolysis (EN)

Εθνικό Μετσόβιο Πολυτεχνείο (EL)
National Technical University of Athens (EN)

Biotechnology Letters (EN)

English

1997


CHAPMAN HALL LTD (EN)



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