Coordination of Cu(2+) and Ni(2+) with the histone model peptide of H2B N-terminal tail (1-31 residues): A spectroscopic study

 
This item is provided by the institution :
University of Ioannina
Repository :
Repository of UOI Olympias
see the original item page
in the repository's web site and access all digital files if the item*
share



2010 (EN)
Coordination of Cu(2+) and Ni(2+) with the histone model peptide of H2B N-terminal tail (1-31 residues): A spectroscopic study (EN)

Nunes, A. M. (EN)

Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας (EL)
Nunes, A. M. (EN)

The interaction of Cu(2+) and Ni(2+) with the N-terminal tail of histone H2B, the 31 amino acid peptide H2B(1-31) (Ac-PEPAKSAPAPKKG(13)SKKAVTKAQKKD(25)GKKRKR-NH(2)), studied by various spectroscopic techniques (UV/Vis, CD, EPR and NMR) are described. The results showed the formation of Cu(2+)-H2B(1-31) complexes above pH 7.3 most probably through the beta-carboxylate group of D25. With the increase of the pH, a mixture of 3 N and 4 N species presenting {2N(-), CO, epsilon NH(2)} and {2N(-), OH(2), epsilon NH(2)}{epsilon NH(2)} coordination modes, respectively is formed, while at highly basic solutions the binding of an additional amide donor is suggested. NMR spectroscopy supported by CD spectroscopy indicated that Ni(2+) coordination takes place most likely through Q22-K23-K24-D25 peptide fragment. Direct coordination to Ni(2+), in a {4N(-)} coordination mode, with a severe conformation change in all residues from G13 to G26 was observed. Cu(2+) and Ni(2+) binding to the N-terminal tail of H2B causes a severe conformational change that might interfere with histone post-translational modi. cations, possibly leading to epigenetic changes. (EN)

protein secondary structure (EN)

Πανεπιστήμιο Ιωαννίνων (EL)
University of Ioannina (EN)

Dalton Transactions (EN)

2010

<Go to ISI>://000277031800016

Royal Society of Chemistry (EN)



*Institutions are responsible for keeping their URLs functional (digital file, item page in repository site)