Determination of hydrogen peroxide by using a flow injection system with immobilized peroxidase and long pathlength capillary spectrophotometry

 
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2002 (EN)
Determination of hydrogen peroxide by using a flow injection system with immobilized peroxidase and long pathlength capillary spectrophotometry (EN)

Pappas, A. C. (EN)

Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας (EL)
Pappas, A. C. (EN)

The development of a highly sensitive method for the determination of nanomolar concentrations of hydrogen peroxide in the liquid phase is described. This paper demonstrates for the first time a flow injection analysis (FIA) system with immobilized enzyme reactor combined with a total internal reflective cell (a liquid waveguide capillary cell (LWCC)) and spectrophotometric detection, for the development of an improved procedure for the determination of hydrogen peroxide. Moreover, the newly synthesized 4-aminopyrazolone derivative, 4-amino-5-(p-aminophenyl)-1-methyl-2-phenyl-pyrazol-3-one (DAP), is used as a color coupler in its oxidative condensation with the sodium salt of N-ethyl-N-sulphopropylaniline sodium salt (ALPS) which acts as a hydrogen donor. Immobilization of peroxidase is achieved by coupling the periodate-treated enzyme to aminopropyl controlled-pore glass (CPG) beads. The determination of hydrogen peroxide is carried out in a 0.1 M phosphate buffer and the product is monitored at 590 nm with a charge-coupled device (CCD) detector equipped with fiber optics in a fully computerized system. The interference of different species, mainly ionic, was investigated. The method permits detection down to 4 nmol l(-1) hydrogen peroxide (signal-to-noise ratio = 3). A linear calibration graph was obtained over the range 20-700 nmol l(-1). The relative standard deviation (R.S.D.) at 300 nmol l(-1) H,02 is 1.7% (n = 7), The method was successfully applied for the determination of hydrogen peroxide in samples from a vat-cleaning process. (C) 2002 Elsevier Science B.V. All rights reserved. (EN)

hydrogen peroxide (EN)

Πανεπιστήμιο Ιωαννίνων (EL)
University of Ioannina (EN)

Analytica Chimica Acta (EN)

2002

<Go to ISI>://000174586200015

Elsevier (EN)



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