Cpp, an Nmda-Receptor Antagonist, Blocks 4-Aminopyridine-Induced Spreading Depression Episodes but Not Epileptiform Activity in Immature Rat Hippocampal Slices

 
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1992 (EN)
Cpp, an Nmda-Receptor Antagonist, Blocks 4-Aminopyridine-Induced Spreading Depression Episodes but Not Epileptiform Activity in Immature Rat Hippocampal Slices (EN)

Psarropoulou, C. (EN)

Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών και Τεχνολογιών. Τμήμα Βιολογικών Εφαρμογών και Τεχνολογιών (EL)
Psarropoulou, C. (EN)

Spontaneous episodes of spreading depression (SD) were observed in the CA3 subfield of immature or young (2-30 days postnatally) hippocampal slices perfused with medium containing 4-aminopyridine (4-AP, 50-mu-M). SD appeared in 34% of the hippocampal slices examined and was more frequently observed in slices obtained from 11 to 20-day-old animals. SD studied with extracellular field potential recordings consisted of large amplitude (18.7 +/- 1.1 mV, mean +/- S.E.M.) negative DC shifts that lasted 30-250 s. Unlike the epileptiform activity that was concomitantly seen during 4-AP application, SD was blocked by the NMDA receptor antagonist 3-((RS)-2-carboxypiperazine-4-yl)-propyl-l-phosphonic acid (CPP, 2-10-mu-M). In contrast, 6-cyano-7-nitro-quinoxaline-2,3-dione (CNQX, 5-mu-M), a non-NMDA-type receptor antagonist, blocked the epileptiform activity but only increased the interval between SD episodes. These results demonstrate that immature hippocampal tissue is susceptible to SD episodes, when perfused with 4-AP-containing medium, and that the occurrence of these episodes presumably depends on the activation of the NMDA receptor. In addition these findings indicate that SD shows a sensitivity to excitatory amino acid receptor antagonists that differs from that of the epileptiform activity recorded simultaneously. (EN)

immature hippocampus (EN)

Πανεπιστήμιο Ιωαννίνων (EL)
University of Ioannina (EN)

Neurosci Lett (EN)

English

1992

<Go to ISI>://A1992HC66200034



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