Gas chromatographic determination of carbonyl compounds in biological and oil samples by headspace single-drop microextraction with in-drop derivatisation
Gas chromatographic determination of carbonyl compounds in biological and oil samples by headspace single-drop microextraction with in-drop derivatisation
(EN)
Fiamegos, Y. C.
(EN)
Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας
(EL)
Fiamegos, Y. C.
(EN)
A suitable method for the gas chromatographic determination of 10 characteristic carbonyls in biological and oil samples based on the in-drop formation of hydrazones by using 2,4,6-trichlorophenylhydrazine (TCPH), has been developed. The derivatisation-extraction procedure was optimized separately for aqueous and oil samples with respect to the appropriate organic drop solvent, drop volume, in-drop TCPH concentration, sample stirring rate, temperature during single-drop microextraction (SDME), reaction time and headspace-to-sample volume ratio. The optimization showed differentiation of optimum values between the studied matrices. The limits of detection were found to range from 0.001 to 0.003 mu g mL(-1) for the aqueous biological samples and from 0.06 to 0.20 mu g mL(-1) for the oil samples. The limits of quantification were in the range of 0.003-0.010 mu g mL(-1) and 0.020-0.059 mu g mL(-1) for aqueous and oil samples, respectively The overall relative standard deviations of the within-day repeatability and between-day reproducibility were < 4.4% and < 8.2% for the aqueous biological samples and < 3.9% and < 7.4% for the oxidized oil samples. (c) 2008 Elsevier B.V. All rights reserved.
(EN)
