Hybridization and cellular uptake properties of lipophilic oligonucleotide-dendrimer conjugates
(EN)
Skobridis, K.
(EN)
Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας
(EL)
Skobridis, K.
(EN)
Dendrimeric compounds were conjugated to oligonucleotides in order to improve their cellular uptake. Second and third generation lipophilic dendrimers were covalently attached either to the 5'- or 3'- end of oligonucleotides. Thermal denaturation experiments showed that the attachment of a third generation dendrimer leads to a substantial decrease in binding affinity. The considerably smaller second-generation dendrimers, however, are well tolerated. Fluorescence measurements revealed that the presence of a second-generation dendrimer leads to a marked increase in the cellular uptake of oligonucleotides.
(EN)
