NMR evidence for major grove binding of both Lambda- and Delta-[Ru(bpy)(2)(pqx)](PF6)(2) to the oligonucleotide d(CGCGAATTCGCG)(2)
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Garoufis, A.
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Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας
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Garoufis, A.
(EN)
The enantiomeric complexes Lambda- and Delta-[Ru(bpy(2)(pqx)](PF6)(2) [pqx = 2-(2'-pyridyl)quinoxaline] were synthesized and characterized by various spectroscopic techniques. Their binding properties to the oligonucleotide duplex d(CGCGAATTCGCG)(2) were subsequently studied by NMR spectroscopy. 1D and 2D NMR techniques provided evidence for major-groove binding of both enantiomers with the oligonucleotide. The Delta-[Ru(bpy)(2)(pqx)](PF6)(2) enantiomer binds more deeply enantiomer to the oligonucleotide than the Lambdaone. The quinoxalinic moiety of the pqx ligand faces the major groove in the Delta complex and is oriented at the external side of the adduct in the Lambda complex. Both complexes show some affinity for the T8C9 base sequence of the dodecanucleotide helix. (C) Wiley-VCH Verlag GmbH Co.
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