Proteolytic activities of Leuconostoc FC9 isolated from fetal cheese
(EN)
Lambropoulos, I.
(EN)
Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας
(EL)
Lambropoulos, I.
(EN)
The growth of Leuconostoc FC9 isolated from Feta cheese in media containing casein, casein hydrolysate or cheese water-soluble extract as sole nitrogen source was studied. FC9 grew fast in media of soluble nitrogen materials, and to some extent in casein media. In the presence of 4 % NaCl, FC9 grew to some extent in casein hydrolysate, while its growth in casein was very low. The cell-envelope extract of FC9 hydrolysed both alpha- and beta-caseins, faster the latter, as indicated by SDS-PAGE and RP-HPLC. The cell-free extract of FC9 hydrolysed both alpha- and beta-caseins, while their hydrolysis was significantly inhibited by 4% NaCl as indicated by SDS-PAGE. The ability of Leuconostoc FC9 to assimilate casein and cheese soluble nitrogenous materials along with the ability of its enzymes to hydrolyse caseins indicates that the FC9 strain may have an impact on cheese proteolysis.
(EN)
