Reagentless enzyme electrode for malate based on modified polymeric membranes
(EN)
Maines, A.
(EN)
Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας
(EL)
Maines, A.
(EN)
A series of polymeric membranes have been employed as outer barriers in an amperometric malate dehydrogenase (MDH)/diaphorase (DI) or MDH/DI/NAD(+)/mediator enzyme electrode for the determination of malate in undiluted neutral or acidic media. MDH/DI were physically entrapped with NAD(+) and mediator in a mixed ester cellulose membrane. Outer membranes such as non-anionic surfactant-modified cellulose acetate/Tween-80 and unplasticised spin coated PVC/polycarbonate (PC) resin in conjunction with an ascorbate oxidase (AOD) layer were utilised. Mechanical strength, thickness studies and diffusional properties of the membranes were investigated. Hexacyanoferrate(III), 2,4-dichlorophenolindophenol (DCPI) and naphthoquinone (NQ) were tested as mediators for enzymatically produced NADH using cyclic voltammetry. Analytical utility of the sensors is demonstrated. (C) 2000 Elsevier Science B.V. All rights reserved.
(EN)
