Simultaneous determination of phenolic compounds and tocopherols in virgin olive oil using HPLC and UV detection

 
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2001 (EN)
Simultaneous determination of phenolic compounds and tocopherols in virgin olive oil using HPLC and UV detection (EN)

Tasioula-Margari, M. (EN)

Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας (EL)
Tasioula-Margari, M. (EN)

A simple procedure is described for I he simultaneous extraction and HPLC determination of phenols and tocopherols in virgin olive oil. The extraction was carried out using methanol and an isopropanol- methanol mixture. Separation was achieved on a reversed phase C-18 column with acetic acid/water-methanol-acetonitrile-isopropanol mixture under gradient elution. Detection was accomplished with UV detection at lambda==280 nm. Using this method, simple and complex phenols as well as alpha -tocopherol can be determined in one run. Phenolic compounds were totally extracted whereas an average recovery of 80% was achieved for alpha -tocopherol. Validation of method is reported for olive oil samples with varying concentrations of alpha -tocopherol and phenolic compounds. (C) 2001 Elsevier Science Ltd. All rights reserved. (EN)

virgin olive oil (EN)

Πανεπιστήμιο Ιωαννίνων (EL)
University of Ioannina (EN)

Food Chemistry (EN)

English

2001

<Go to ISI>://000170507600015

Elsevier (EN)



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