Synthesis and characterization of ruthenium(II)-oligopyridine-peptide conjugates. Interactions of the diasteromeres Delta- and Lambda-[Ru(bpy)(2) (4-COY-4 '-Mebpy)]Cl-2 (Y = Gly-Lys(1)-Lys(2)CONH(2), Lys(1)-Gly-Lys(2)CONH(2), Lys(1)-Lys(2)-GlyCONH(2)) with the oligonucleotide d(5 '-CGCGAATTCGCG-3 ')(2)

 
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Synthesis and characterization of ruthenium(II)-oligopyridine-peptide conjugates. Interactions of the diasteromeres Delta- and Lambda-[Ru(bpy)(2) (4-COY-4 '-Mebpy)]Cl-2 (Y = Gly-Lys(1)-Lys(2)CONH(2), Lys(1)-Gly-Lys(2)CONH(2), Lys(1)-Lys(2)-GlyCONH(2)) with the oligonucleotide d(5 '-CGCGAATTCGCG-3 ')(2) (EN)

Karidi, K. (EN)

Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας (EL)
Karidi, K. (EN)

Diastereomeric complexes of the general formulae Lambda- and Delta-[Ru(bpy)(2)(4-COY-4'-Mebpy)]Cl-2 where bpy = 2,2'-bipyridine and Y = Gly-Lys(1)-Lys(2)CONH(2), Lys(1)-Gly-Lys(2)CONH(2), Lys(1)-Lys(2)-GlyCONH(2), were synthesized and characterized. The ability of these compounds to bind to the oligonucleotide duplex d(5'-CGCGAATTCGCG-3') was studied with NMR techniques. Complex Lambda-2, Lambda-[Ru(bpy)(2)(4-COLys(1)-Gly-Lys(2)CONH(2)),4'-Mebpy)]Cl-2 (Mebpy = methyl-2,2'-bipyridine), interacts non-specifically causing changes for both complex and oligonucleotide H-1 NMR signals. Both Lambda-1, Lambda-[Ru(bpy)(2)(4-COGly-Lys(1)-Lys(2)CONH(2)),4'-Mebpy)]Cl-2 and Lambda-3, Lambda-[Ru(bpy)(2)(4-COLys(1)-Lys(2)-GlyCONH(2)),4'-Mebpy)]Cl-2, were bound to the oligonucleotide through both lysine aliphatic chains, indicating that the side chains of the sequential lysines create a kind of "clamp" to connect the complex with the oligonucleotide. Complex Delta-1, Delta-[Ru(bpy)(2)(4-COGly-Lys(1)-Lys(2)CONH(2)),4'-Mebpy)]Cl-2, interacts with the oligonucleotide duplex with both lysine side chains in a manner similar to Lambda-1. Delta-2, Delta-[Ru(bpy)(2)(4-COLys(1)-Gly-Lys(2)CONH(2)),4'-Mebpy)Cl-2, interacts with the oligonucleotide with the bipyridine ligands. In addition, the formation of a hydrogen bond between the Gly-NH and the carbonyl groups of the oligonucleotide bases was detected. A completely different binding mode was observed for Delta-3 Delta-[Ru(bpy)(2)(4-COLys(1)-Lys(2)-GlyCONH(2)),4'-Mebpy)]Cl-2, which at a ratio of 1:1 ([Ru]/[nucleotide]) opens the oligonucleotide strands. In addition, participation of all three peptidic NH of Delta-3 in hydrogen bonds was observed. (c) 2013 Elsevier Inc. All rights reserved. (EN)

ruthenium (EN)

Πανεπιστήμιο Ιωαννίνων (EL)
University of Ioannina (EN)

J Inorg Biochem (EN)

Αγγλική γλώσσα

2013

<Go to ISI>://000324661500003

Elsevier (EN)



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