Synthesis and characterization of the diastereomers Lambda- and Delta-[Ru(bpy)(2)-(m-bpy-Gly-L-His-L-Lys)]Cl-2 - H-1 NMR studies on their interactions with the deoxynucleotide duplex d[(5 '-CGCGAATTCGCG-3 ')(2)]

 
This item is provided by the institution :
University of Ioannina
Repository :
Repository of UOI Olympias
see the original item page
in the repository's web site and access all digital files if the item*
share



2004 (EN)
Synthesis and characterization of the diastereomers Lambda- and Delta-[Ru(bpy)(2)-(m-bpy-Gly-L-His-L-Lys)]Cl-2 - H-1 NMR studies on their interactions with the deoxynucleotide duplex d[(5 '-CGCGAATTCGCG-3 ')(2)] (EN)

Myari, A. (EN)

Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας (EL)
Myari, A. (EN)

The synthesis and characterization of the diastereomeric complexes Lambda- and Delta-[Ru(bpy)(2)(m-bpy-GHK)]Cl-2, (GHK = glycine-L-histidine-L-lysine, m-bpy = 4\-methyl-2,2\-bipyridine) as well as their binding properties to the deoxynucleotide duplex d(5'-CGCGAATTCGCG-3')2 studied by means of NMR, ESI-MS and CD spectroscopy, are reported. The ROESY spectrum of Lambda-[Ru(bpy)(2)(m-bpy-GHK)]Cl-2 shows intramolecular cross peaks between the bpy H3 or HT protons and the aromatic H2 and H5 of the histidine imidazole ring, indicating that the peptide adopts an orientation with the imidazole ring close to the bpy ligand, possibly interacting by pi-stacking. The absence of intramolecular cross peaks between the peptide and the bipyridine ligands in the ROESY spectrum of Delta-[Ru(bpy)(2)(m-bpy-GHK)]Cl-2 on the other hand, shows that in this case the peptide is far from the two bpy ligands, having a different orientation from the A isomer. The isomers interact with the oligonucleotide duplex differently. Delta-[Ru(bpy)(2)(m-bpy-GHK)]Cl-2 binds in the oligonucleotide major groove close to the central part of the sequence. A[Ru(bpy)(2)(m-bpy-GHK)]Cl-2 binds, probably non selectively, approaching the helix from the minor groove. It can be concluded that the peptide (GHK) binding leads the rest of the isomer to interact with the oligonucleotide in the case of the A isomer. (C) Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2004. (EN)

metallopeptides (EN)

Πανεπιστήμιο Ιωαννίνων (EL)
University of Ioannina (EN)

European Journal of Inorganic Chemistry (EN)

English

2004

<Go to ISI>://000220797100009

Wiley-VCH Verlag (EN)



*Institutions are responsible for keeping their URLs functional (digital file, item page in repository site)