Synthesis and characterization of the diastereomers Lambda- and Delta-[Ru(bpy)(2)(m-bpy-L-Arg-Gly-L-Asn-L-Ala-L-His-L-Glu-L-Arg)]Cl-2 H-1 NMR studies on their interactions with the deoxynucleotide duplex d[(5 '-GCGCTTAAGCGC-3 ')(2)] and d[(5 '-CGCGATCGCG-3 ')(2)] (EN)

Myari, A. (EN)

Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας (EL)

Myari, A. (EN)

The diastereomeric complexes Lambda- and Delta-[Ru(bpy)(2)(M-bpy-7p)]Cl-2, (bpy = 2,2'-bipyridine, m-bpy-7p = 4-methyl-4'-Arg-Gly-Asn-Ala-His-Glu-Arg-CONH2-2,2'-bipyridine) were synthesized and characterized and their binding properties to the deoxynucleotide duplexes d(5'-CGCGATCGCG-3')(2) and d(5'-GCGCTTAAGCGC-3')(2) were studied by means of H-1 NMR spectroscopy. 7p is part of the recognition loop of the restriction endonuclease MunI, a type II restriction enzyme from Mycoplasma unidentified which recognizes the palindromic hexanucleotide sequence C/AATTG and cleaves it as indicated by the slash. The A-isomer binds to the terminal CG/GC major groove of d(CGCGATCGCG)(2) decanucleotide, whereas the A-isomer approaches the GCT/CGA sequence. On the other hand, weak binding of the Delta-isomer to the end of d(GCGCTTAAGCGC)(2) into two different orientations is observed. In the case of the A-isomer, the bpy ligand(s) are located into the major groove of the central TT/AA sequence. The role of appended peptide sequences in sequence selectivity binding to DNA is being addressed. (C) 2004 Elsevier Inc. All rights reserved. (EN)

metallopeptides (EN)

Πανεπιστήμιο Ιωαννίνων

Ιδρυματικό Αποθετήριο Ολυμπιάς

Σχολή Θετικών Επιστημών

Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

ΑΠΟΘΕΤΗΡΙΟ "ΟΛΥΜΠΙΑΣ"

Τμήμα Χημείας

J Inorg Biochem (EN)

Αγγλική γλώσσα

2005

<Go to ISI>://000226392400031

http://olympias.lib.uoi.gr/jspui/handle/123456789/9409

Elsevier (EN)