Identification and discrimination of eight Greek grape cultivars (Vitis vinifera L.) by random amplified polymorphic DNA markers
Hatzopoulos, Polidefkis
Biniari, Katerina
Stavrakakis, Manolis
Vitis, 36 (4) 175-178
Fifteen decamer primers of an arbitrary nucleotide sequence were used to amplify genomic DNA by polymerase chain reaction (PCR-RAPD) in order to identify and discriminate between 8 cultivars of Vitis vinifera L., grown at the Island of Crete. Over 140 reproducible polymorphic fragments were generated by this method. Each grape cultivar showed a unique banding pattern for more than 5 of the primers used. Herefrom, the degree of genetic similarity was calculated and the dendrogram of the 8 cultivars was constructed. The results show that RAPD is a reliable and very useful method for the identification and genomic analysis of grape cultivars .
