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Heat killed Dietzia maris reduces lipopolysaccharide-induced inflammatory responses in murine adherent peritoneal cells

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Heat killed Dietzia maris reduces lipopolysaccharide-induced inflammatory responses in murine adherent peritoneal cells (EL)
Heat killed Dietzia maris reduces lipopolysaccharide-induced inflammatory responses in murine adherent peritoneal cells (EN)
GHAHVECHI-DIZAJI, B.
RIPLEY, P.
HAMIDIAN, G.H.
BARADARAN, B.
SAFARALIZADEH, R.
NOFOUZI, K.
ZARRINI, G.H.
MCINTYRE, G.
MOUSAVI, S.
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
2020-07-01
ΔΕΝ ΔΙΑΤΙΘΕΤΑΙ ΠΕΡΙΛΗΨΗ (EL)
Dietzia maris (D. maris) is a gram-positive, aerobic, mycolic acid-containing actinomycete without mycelium. Actinomycetes such as Tsukamurella inchonensis reduce lipopolysaccharide- induced inflammatory responses in activated murine peritoneal macrophages. Here, the effects of D. maris on LPS-induced inflammatory responses were examined in mouse adherent peritoneal cells. D. maris was grown, harvested, and washed. Suspensions were standardized by wet weight, re-suspended in borate-buffered saline, and autoclaved. For in vivo study, each mouse was orally administered by bacterial suspension (5 × 107, 1 × 108 and 2 × 108 CFU/Mouse) consecutively for seven days. Control animals received the same amount of phosphate-buffered saline (PBS). Adherent peritoneal cells were harvested for in vitro experiments. Cells were lavaged and plated in RPMI 1640 medium, stimulated with LPS (100 ng/ml), and incubated for 2 h. Afterward, non-adherent cells were removed followed by adding freshly prepared medium. Supernatants (50 μl) were collected, centrifuged, mixed with Griess reagent, and the absorbance was measured at 560 nm. D .maris inhibited LPS-stimulated nitric oxide (NO) production in murine macrophages at concentrations of 5 × 107, 1 × 108 and 2 × 108 CFU/Mouse. Also, D. maris decreased LPS-induced production of pro-inflammatory cytokines of interleukin (IL)-6 at all doses. By contrast, tumor necrosis factor (TNF)-α was not effected by D. maris treatment of mice. Our results indicate that D. maris is a potent inhibitor of LPS-induced NO production. D. maris may be useful as a novel agent for the chemoprevention of inflammatory disease. (EN)
Adherent peritoneal cells (EL)
Lipopolysaccharide (EL)
Anti-inflammation (EL)
IL-6 (EL)
Dietzia maris (EL)
Adherent peritoneal cells (EN)
Lipopolysaccharide (EN)
Anti-inflammation (EN)
IL-6 (EN)
Dietzia maris (EN)
Περιοδικό της Ελληνικής Κτηνιατρικής Εταιρείας
English
Hellenic Veterinary Medical Society / Ελληνική Κτηνιατρική Εταιρεία (EN)
1792-2720
2585-3724
Περιοδικό της Ελληνικής Κτηνιατρικής Εταιρείας; Τόμ. 71 Αρ. 2 (2020); 2201-2206 (EL)
Journal of the Hellenic Veterinary Medical Society; Vol. 71 No. 2 (2020); 2201-2206 (EN)
Copyright (c) 2020 K. NOFOUZI, B. GHAHVECHI-DIZAJI, G.H. ZARRINI, B. BARADARAN, G.H. HAMIDIAN, R. SAFARALIZADEH, G. MCINTYRE, P. RIPLEY, S. MOUSAVI (EN)
https://creativecommons.org/licenses/by-nc/4.0
Hellenic Veterinary Medical Society
Journal of the Hellenic Veterinary Medical Society
Research Articles



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