Δισδιάστατη και τρισδιάστατη εναπόθεση βιοσυμβατών και βιολογικών υλικών χρησιμοποιώντας τεχνικές λέιζερ
2D and 3D deposition of biocompatible and biological materials by laser based techniques
In the first part of this thesis, we demonstrate a novel method of the precise, three-dimensional patterning of beta amyloid self-assmbled peptide fibrils, on 3D structures of Ormocer by using the multi-photon polymerization method. The technique which combines femtosecond laser technology and biotin avidin mediated assembly on a polymeric matrix, can be applied in a wide variety of fields, from molecular electronics to tissue engineering.
Molecular self-assembly is emerging as a viable "bottom-up" approach for fabricating nanostructures. Self-assembled biomolecular structures are particularly attractive, due to their versatile chemistry, molecular recognition properties, and biocompatibility. Among them, amyloid protein and peptide fibrils are self-assembled nanostructures with unique physical and chemical stability, formed from quite simple building blocks; their ability to work as a template for the fabrication of low resistance, conducting nanowires has already been demonstrated. The precise positioning of peptide-based nanostructures is an essential part of their use in technological applications, and their controlled assembly, positioning, and integration into microsystems is a problem of considerable current interest. To date, their positioning has been limited to their placement on flat surfaces or to the fabrication of peptide arrays by L.I.F.T. technique.
Over the last few years, there has been a lot of interest and progress in the direct laser writing of biological molecules. Laser induced forward transfer (L.I.F.T.) is a direct-write non contact method, which offers an interesting and versatile alternative to conventional arraying techniques, since it does not require the use of expensive photolithographic equipment, and it does not suffer from clogging and contamination problems, as pin deposition and ink-jet printing do. It is the transfer distances, the precise and high focusing ability of lasers and the laser directionality, which gives flexibility to the L.I.F.T. method. In the second part of this thesis, patterns of different kind of cells were created by using L.I.F.T. method. By using again L.I.F.T. followed the 3D structuring of calcium alginate gel, those structures can be used as a scaffold for tissue engineering. Tissue engineering refers to a special group of radically new procedures and techniques developed for the treatment of various diseases and injuries.