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Spectroscopic and catalytic studies of lipases in ternary hexane–1-propanol–water surfactantless microemulsion systems

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Spectroscopic and catalytic studies of lipases in ternary hexane–1-propanol–water surfactantless microemulsion systems
Ζουμπανιώτη, Μαρία
Παπαδημητρίου, Βασιλική
Ξενάκης, Αριστοτέλης
Stamatis, Haralambos
Άρθρο σε επιστημονικό περιοδικό
2006
A series of water-in-oil microemulsion systems formulated without surfactant were used to solubilize lipases from Rhizomucor miehei and Candida antarctica B. The effect of the system’s composition on the velocity of enzymic reactions was investigated following a model esterification reaction. The interaction between enzymes and the microemulsion environment was studied by steady state fluorescence spectroscopy. The site of localization of the enzyme within the different microdomains of the dispersed phase was investigated by applying the fluorescence energy transfer technique. To determine the properties of the interface between water and organic solvent of the surfactantless microemulsion systems the Electron Paramagnetic Resonance (EPR) spectroscopic technique was applied. The results indicated that even at low water content, water-rich structures are formed. This was confirmed by conductivity measurements. By the addition of enzyme it was observed that when the aqueous phase of the surfactantless microemulsion systems exceeds 2% (v/v) the enzyme retains its catalytic activity, as it is located within the water pools that protect it from the organic solvent. These confined water phases show a propanol rich interface with hexane and their structure depends on the system’s composition.
Επιστήμη (EL)
Science (EN)
Spin probe
Biocatalysis
Microemulsion
Lipase
Energy transfer
English
Elsevier BV
Colloids and Surfaces B: Biointerfaces
https://rightsstatements.org/page/InC/1.0/?language=en
© 2005 Elsevier B.V. All rights reserved
© 2005 Elsevier B.V. All rights reserved (EL)
National Hellenic Research Foundation (NHRF)
Helios - Repository of the National Hellenic Research Foundation (NHRF)



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