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Expression analysis of mir-17-5p, mir-20a and let-7a microRNAs and their target proteins in CD34+ bone marrow cells of patients with myelodysplastic syndromes

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Expression analysis of mir-17-5p, mir-20a and let-7a microRNAs and their target proteins in CD34+ bone marrow cells of patients with myelodysplastic syndromes
Papageorgiou S.G.
Economopoulou C.
Vasilatou D.
Mpakou V.
Pappa V.
Kontos C.K.
Tsiotra P.
Kontsioti F.
Pavlou M.A.S.
Δημητριάδης, Γιώργος
Dervenoulas J.
Άρθρο σε επιστημονικό περιοδικό
2013
Mir-17-5p and mir-20a, members of the mir-17-92 family, down-regulate E2F1, which is over-expressed in myelodysplastic syndromes (MDS). Moreover, let-7a down-regulates KRAS, which is aberrantly expressed in MDS. We evaluated the expression of the aforementioned microRNAs in CD34+ cells of 43 MDS patients using real-time PCR and their target proteins (E2F1, MYC, BCL2, CCND1, and KRAS) by Western blot. Mir-17-5p and mir-20a were under expressed in high risk MDS patients, compared to low risk MDS patients. Similarly, let-7a was under expressed in patients with intermediate or high-risk karyotype. Interestingly, there was an inverse correlation between microRNA and the expression levels of their targets. Importantly, mir-17-5p and mir-20a constitute favorable prognostic factors in MDS, since their expression was associated with increased overall survival of MDS patients.
Βιολογία (Γενικά) (EL)
Γενετική (EL)
Biology (General) (EN)
Genetics (EN)
Mir-20a (EN)
MicroRNAs (EN)
Mir-17-5p (EN)
Let-7a (EN)
MDS (EN)
English
Leukemia Research
© 2012 Elsevier Ltd.
© 2012 Elsevier Ltd. (EN)
National Hellenic Research Foundation (NHRF)
Helios - Repository of the National Hellenic Research Foundation (NHRF)



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