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2007 (EN)
Oleosin gene expression in olive (EN)

Μπανίλας, Γεώργιος Π. (EL)
Χατζόπουλος, Πολυδεύκης (EL)
Γιαννούλια, Κατερίνα Χ. (EL)

Τεχνολογικό Εκπαιδευτικό Ίδρυμα Αθήνας. Σχολή Τεχνολογίας Τροφίμων και Διατροφής. Τμήμα Οινολογίας και Τεχνολογίας Ποτών (EL)

To study the spatial and temporal regulation of oleosin gene expression during olive drupe development, a cDNA (OeOLE) was isolated from embryos. The deduced amino acid sequence of 165 amino acid residues exhibits a long central hydrophobic stretch, including the conserved "proline knot" motif. Phylogenetic analysis grouped OeOLE into the class of high (H) molecular weight oleosins. Southern blotting indicated that the gene is represented by 1-2 copies in the olive genome. Transcript analysis revealed that OeOLE is expressed solely in seeds. A similar bell-shaped pattern of expression was observed in both embryos and endosperms. Transcript accumulation starts at late heart embryo stage, reaches maximum levels at mid-torpedo stage and thereafter declines, coinciding the stages of most oil accumulation in those tissues. (EN)

journalArticle

Olive (EN)
Αναπτυξιακή ρύθμιση (EN)
Developmental regulation (EN)
Ενδοσπέρμιο (EN)
oleosin (EN)
Έμβρυα (EN)
Ελιά (EN)
Ολεοσίνη (EN)
Endosperm (EN)
Embryos (EN)

ΤΕΙ Αθήνας (EL)
Technological Educational Institute of Athens (EN)

Journal of Plant Physiology (EN)

English

2007-01-22

doi:10.1016/j.jplph.2006.03.016

Elsevier GmbH (EN)



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