Antimicrobial activity of acidic xylo-oligosaccharides produced by family 10 and 11 endoxylanases

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Antimicrobial activity of acidic xylo-oligosaccharides produced by family 10 and 11 endoxylanases (EN)

Christakopoulos, P (EN)
Katapodis, P (EN)
Kalogeris, E (EN)
Skaltsa, H (EN)
Macris, BJ (EN)
Stamatis, H (EN)
Kekos, D (EN)

journalArticle (EN)

2014-03-01T01:18:40Z
2003 (EN)


Acidic oligosaccharides were obtained from birchwood xylan by treatment with a Thermoascus aurantiacus family 10 and a Sporotrichum thermophile family I I endoxylanases. The main difference between the products liberated by xylanases of family 10 and 11 concerned the length of the products containing 4-O-methyl-D-glucuronic acid. The xylanase from T aurantiacus liberate from glueuronoxylan an aldotetrauronic acid as the shortest acidic fragment in contrast with the enzyme from S. thermophile, which liberated an aldopentauronic acid. Acidic xylooligosaccharides were separated from the hydrolysate by anion-exchange and size-exclusion chromatography (SEC) and the primary structure was determined by C-13 NMR spectroscopy. The acidic xylooligosaccharides were tested against three Gram-positive and three Gram-negative aerobically grown bacteria, as well as against Helicobacter pylori. Aldopentauronic acid was proved more active against the Gram-positive bacteria and against H. pylori. (C) 2002 Elsevier Science B.V. All rights reserved. (EN)

Biochemistry & Molecular Biology (EN)

Antimicrobial activity (EN)
Negibacteria (EN)
Posibacteria (EN)
Helicobacter (EN)
gel permeation chromatography (EN)
Chromatography, Thin Layer (EN)
oligosaccharide (EN)
carbohydrate analysis (EN)
Thermoascus aurantiacus (EN)
aldopentauronic acid (EN)
Xylans (EN)
carbohydrate synthesis (EN)
Magnetic Resonance Spectroscopy (EN)
birch (EN)
nuclear magnetic resonance spectroscopy (EN)
Thermoascus (EN)
xylan (EN)
Oligosaccharides (EN)
Acidic xylo-oligosaccharides (EN)
aerobic bacterium (EN)
article (EN)
uronic acid (EN)
10 endoxylanase (EN)
xylooligosaccharide (EN)
nonhuman (EN)
unclassified drug (EN)
11 endoxylanase (EN)
Dose-Response Relationship, Drug (EN)
Anti-Bacterial Agents (EN)
Helicobacter pylori (EN)
Sporotrichum (EN)
glucuronic acid (EN)
Xylan Endo-1,3-beta-Xylosidase (EN)
Xylosidases (EN)
Sporothrix (EN)
Uronic Acids (EN)
4 o methyl dextro glucuronic acid (EN)
anaerobic bacterium (EN)
glucuronoxylan (EN)
bacterial enzyme (EN)
antimicrobial activity (EN)
bacterial growth (EN)
Chromatography (EN)
Xylanases (EN)
Hydrolysis (EN)
Corynascus heterothallicus (EN)
controlled study (EN)
aldotetrauronic acid (EN)
anion exchange (EN)
Endo-1,4-beta Xylanases (EN)

International Journal of Biological Macromolecules (EN)

English

ELSEVIER SCIENCE BV (EN)




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