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In vitro three-stage continuous fermentation of wheat arabinoxylan fractions and induction of hydrolase activity by the gut microflora

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National Technical University of Athens   

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In vitro three-stage continuous fermentation of wheat arabinoxylan fractions and induction of hydrolase activity by the gut microflora (EN)
Christakopoulos, P (EN)
Gasson, M (EN)
Narbad, A (EN)
Vardakou, M (EN)
Nueno Palop, C (EN)
journalArticle (EN)
2014-03-01T01:26:28Z
2007 (EN)
In vitro fermentations were carried out by using a model of the human colon to stimulate microbial activities of gut bacteria. The model consisted of a three-stage culture system. Bacterial populations were evaluated under the effect of three types of arabinoxylan, a nonstarch polysaccharide, derived from wheat, the water-unextractable arabinoxylan fraction (WU-AX), WU-AX pretreated with exogenous xylanase and the soluble water-extractable arabinoxylan fraction (WE-AX). The xylanase pretreated (WU-AX) had a stimulatory effect upon colonic bifidobacteria throughout all three vessels. Counts of Bacteroides spp. and Clostridium spp. were also both significantly reduced. Addition of the WU-AX substrates to the first vessel resulted in induction of bacteria] synthesis of extracellular hydrolytic enzymes xylanase and ferulic acid esterase which are both required for bacterial metabolism of WU-AX; this induction was significantly greater with the xylanase treated WU-AX. (C) 2007 Elsevier B.V. All rights reserved. (EN)
Biochemistry & Molecular Biology (EN)
Clostridium (EN)
enzyme synthesis (EN)
DNA, Ribosomal (EN)
Bacteroides sp. (EN)
continuous fermentation (EN)
Humans (EN)
ferulic acid (EN)
Triticum (EN)
arabinoxylan (EN)
enzyme activity (EN)
intestine flora (EN)
esterase (EN)
wheat (EN)
Three-stage in vitro colon model (EN)
RNA, Ribosomal, 16S (EN)
Xylans (EN)
Intestinal Mucosa (EN)
xylan endo 1,3 beta xylosidase (EN)
Bifidobacterium (EN)
Ferulic acid esterases (EN)
Flour (EN)
DNA Primers (EN)
Fermentation (EN)
prebiotic agent (EN)
article (EN)
bacterial count (EN)
Xylanases (EN)
Bacteroides (EN)
bacterial metabolism (EN)
enzyme induction (EN)
hydrolase (EN)
Bacteria (microorganisms) (EN)
Intestine, Large (EN)
Hydrolases (EN)
nonhuman (EN)
Kinetics (EN)
Endo-1,4-beta Xylanases (EN)
Triticum aestivum (EN)
Arabinoxylan (EN)
International Journal of Biological Macromolecules (EN)
English
ELSEVIER SCIENCE BV (EN)
National Technical University of Athens
Digital Library of National Technical University of Athens | Dspace@NTUA
Κεντρική Βιβλιοθήκη Ε.Μ.Π.
Ιδρυματικό Αποθετήριο
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