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Comparative qualitative and quantitative analysis of scleroderma (systemic sclerosis) serologic immunoassays

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Εθνικό Μετσόβιο Πολυτεχνείο   

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Comparative qualitative and quantitative analysis of scleroderma (systemic sclerosis) serologic immunoassays (EN)
Moutsopoulos, HM (EN)
Pakas, I (EN)
Picha, L (EN)
Vlachoyiannopoulos, PG (EN)
Guialis, A (EN)
journalArticle (EN)
2014-03-01T01:57:20Z
2008 (EN)
The heterogeneity of autoantibody specificities occurring in sera from patients with systemic sclerosis (SSc) raised the necessity of developing various methodologies for their detection. A cohort of 150 SSc patients were selected and tested by Indirect Immunofluorescence (IIF), Counterimmunoelectrophoresis (CIE), Immunoblot (IB) using various extracts as antigenic source and RNA precipitation. By preparing a nuclear (IB-nuclear) and a metaphase chromosomal-enriched extract (IB-MC-pellet) from HeLa cells as well as a nucleolar (IB-nucleolar) and a histone (IB-histone) extract from rat liver nuclei, we assessed their sensitivity and specificity for anti-Topo 1, anti-U3RNP, anti-H-1, anti-snRNPs antibodies and ACA. IB-nuclear revealed the highest frequency of anti-Topo I antibodies, while CIE, IB-nucleolar and IB-MC-pellet, when compared to IB-nuclear showed a sensitivity of 89%, 87% and 95%, respectively. IB-MC-pellet was unique for ACA recognition, while IB-nucleolar and IB-MC-pellet showed excellent sensitivity for anti-U3RNP and anti-H, antibody detection. We conclude that IB-nuclear is a highly sensitive system for anti Topo I antibodies determination, but CIE reveals a good sensitivity to be used as a first screening test. IB-nucleolar or IB-MC-pellet are important techniques to detect the variety of antibodies to nucleolus and chromatin-related constituents. A novel specificity against a 28 kD nucleolar protein, non-associated with RNAs is also presented. (C) 2008 Elsevier Ltd. All rights reserved. (EN)
Immunology (EN)
Systemic sclerosis (scleroderma) (EN)
RIBONUCLEOPROTEIN PARTICLE (EN)
Autoantibodies (EN)
PULMONARY-HYPERTENSION (EN)
LINKED-IMMUNOSORBENT-ASSAY (EN)
CLINICAL ASSOCIATIONS (EN)
ANTICENTROMERE ANTIBODIES (EN)
LUPUS-ERYTHEMATOSUS (EN)
POLYMYOSITIS OVERLAP (EN)
Immunoblot (EN)
ANTIHISTONE ANTIBODIES (EN)
TOPOISOMERASE-I SCL-70 (EN)
Antigenic source (EN)
NUCLEOLAR ANTIBODIES (EN)
JOURNAL OF AUTOIMMUNITY (EN)
English
ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD (EN)
Εθνικό Μετσόβιο Πολυτεχνείο
Ψηφιακό Αποθετήριο της Κεντρικής Βιβλιοθήκης του ΕΜΠ | Dspace@NTUA
Κεντρική Βιβλιοθήκη Ε.Μ.Π.
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