The Potential Role of RT-LAMP in SARS-CoV-2 Diagnosis Based on Limit of Detection

 
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Open Schools Journal for Open Science  | ΕΚΤ eJournals
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Τμήμα περιοδικού (EL)
2022 (EL)
The Potential Role of RT-LAMP in SARS-CoV-2 Diagnosis Based on Limit of Detection (EN)
Celebi, Ayse
The COVID-19 pandemic, caused by the novel coronavirus SARS-CoV-2, that arose in 2019 underscored the significance of testing and diagnostics in order to combat the rapid spread of the disease. The most common method for SARS-CoV-2 RNA detection has been the RT-qPCR method, which is insufficient in terms of meeting the immense demand for testing due to its relatively high time-consumption. Under these circumstances, the urgent need for a rapid and similarly sensitive testing arised, and the RT-LAMP method was proven to be a promising alternative. This review compares the lowest concentration levels of SARS-CoV-2 RNA that these two mentioned methods can detect, examining several reviews and research. It was found that the detection limit of RT-qPCR is lower in comparison to RT-LAMP, which demonstrates a higher sensitivity, thus, reducing the false negative outcomes. Nonetheless, RT-LAMP is more convenient for being deployed as a point-of-care test for vast amounts of people considering its rapidity in detection and simplicity, albeit it has a higher detection limit compared to RT-qPCR. (EN)
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
RT-LAMP (EN)
LOD (EN)
COVID-19 testing (EN)
RT-qPCR (EN)
Ελληνογερμανική Αγωγή
Open Schools Journal for Open Science
Open Schools Journal for Open Science
Αγγλική γλώσσα
2022-12-20
https://ejournals.epublishing.ekt.gr/index.php/openschoolsjournal/article/view/25422
Ellinogermaniki Agogi (EN)
2623-3606
Open Schools Journal for Open Science; Vol. 5 No. 2 (2022): Open Schools Journal for Open Science (EN)
http://creativecommons.org/licenses/by-nc-sa/4.0 (EN)
Copyright (c) 2022 Ayse Celebi (EN)



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