Surveillance study on campylobacter infestation in poultry

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Μελέτη του επιπολασμού των καμπυλοβακτηριδίων στα πουλερικά
Surveillance study on campylobacter infestation in poultry

Ζησίδης, Νικόλαος
Zisides, Nikolaos

PhD Thesis

2011


Campylobacter spp. particularly C. jejuni has been recognized as one of the most important causes of foodborne bacterial diarrhea in humans worldwide. Since poultry are considered the major reservoir of this foodborne pathogen, the prevalence of Campylobacter spp. in poultry operations is a matter of concern. However, relatively little information on the prevalence of Campylobacter in poultry in Greece is available. Hence, a six year (2005-2010) surveillance study on 369 samples of end-product (chicken breast or leg) was conducted to determine the prevalence of Campylobacter isolates from locally produced poultry. Σhe analysis of the chicken meat samples was performed using an Enzyme Linked Fluorescence Assay and the mini-VIDAS automated system (BioMerieux). Following homogenization in stomacher, enrichment in Bolton Broth and incubation in microaerophilic environment the samples were tested for the presence of Campylobacter using the Vidas CAM strips and reagents. Confirmation and identification to the species level was performed by plating onto CampyFood Agar, incubation at 41.5νC - 42νC for 24-48 hrs and biochemical identification by API Campy. Although Campylobacter in the examined poultry samples was initially highly prevalent (50%) by the end of this survey decreased to 18.51%. The most prevalent species were C. jejuni (59,5%) and C. coli (38,7%), while C. lari prevalence was very low (1,8%). Σhis study further highlights the need for prudent measures to prevent the occurrence and transmission of Campylobacter from the contaminated poultry reservoir to humans through the food-chain

Επιστήμες Υγείας
Ιατρική και Επιστήμες Υγείας

Τρόφιμα, Ασφάλεια
Επιπολασμός των καμπυλοβακτηριδίων
Επιστήμες Υγείας
Medical and Health Sciences
Βακτηριακές τροφιμογενείς λιμώξεις
Prudent measures to prevent the occurrence and transmission of campylobacter
Κοτόπουλα, Κρεοπαραγωγά
Αξιολόγηση του πιθανού κινδύνου για την δημόσια υγεία
Foodborne bacterial diarrhea in humans worldwide
Ιατρική και Επιστήμες Υγείας
Surveillance study on campylobacter infesstation in poultry
Health Sciences

Greek

Πανεπιστήμιο Ιωαννίνων
University of Ioannina

Πανεπιστήμιο Ιωαννίνων. Σχολή Ιατρικής. Τμήμα Ιατρικής. Τομέας Λειτουργικός - Κλινικοεργαστηριακός. Εργαστήριο Μικροβιολογίας




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