Fast screening of municipal wastewater components by reversed-phase chromatography coupled to ultraviolet-diode array detection

 
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Fast screening of municipal wastewater components by reversed-phase chromatography coupled to ultraviolet-diode array detection (EN)

Vlessidis, A. G. (EN)

Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας (EL)
Vlessidis, A. G. (EN)

The screening of ingredients in municipal wastewater samples was studied, by reverse-phase high-performance liquid chromatography (RP-HPLC) implemented with a ultraviolet (UV)-diode array detector, in an effort to establish a quick screening method for finding the main UV-absorbing components of the wastewater that enters the biological treatment plant. For the purposes of direct comparison of the chromatograms obtained, a single sample taken from the inlet of municipal biological treatment plant was used for all experimental tests and the optimal elution conditions were established by detailed investigation through isocratic and gradient elution techniques. The optimal column resolution was reached by testing a broad range of capacity and selectivity factor values. The criterion used for validating the complete separation of solutes was based on the spectral purity of eluted chromatogram peaks and on the stability of retention time of a peak in real-time chromatograms taken at different wavelengths during the elution process. From this research work, it was found that the overall chromatogram peak pattern was sensitive to the mobile phase composition and solvent system but under fixed elution conditions, the pattern of the major peaks was stable and depended on the composition of the wastewater sample. By testing different types of elution modes, it was found that the best column resolution was obtained under programmed isocratic-gradient elution using a binary mobile phase. Finally, a relatively high column resolution method is reported for fragmentation of municipal wastewater samples. (EN)

isocratic-gradient elution (EN)

Πανεπιστήμιο Ιωαννίνων (EL)
University of Ioannina (EN)

Water and Environment Journal (EN)

Αγγλική γλώσσα

2007

<Go to ISI>://000246626500010

Wiley-Blackwell (EN)



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