Synthesis and characterization of the diastereomers Lambda- and Delta-[Ru(bpy)(2)(m-bpy-L-Arg-Gly-L-Asn-L-Ala-L-His-L-Glu-L-Arg)]Cl-2 H-1 NMR studies on their interactions with the deoxynucleotide duplex d[(5 '-GCGCTTAAGCGC-3 ')(2)] and d[(5 '-CGCGATCGCG-3 ')(2)]

 
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Synthesis and characterization of the diastereomers Lambda- and Delta-[Ru(bpy)(2)(m-bpy-L-Arg-Gly-L-Asn-L-Ala-L-His-L-Glu-L-Arg)]Cl-2 H-1 NMR studies on their interactions with the deoxynucleotide duplex d[(5 '-GCGCTTAAGCGC-3 ')(2)] and d[(5 '-CGCGATCGCG-3 ')(2)] (EN)

Myari, A. (EN)

Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας (EL)
Myari, A. (EN)

The diastereomeric complexes Lambda- and Delta-[Ru(bpy)(2)(M-bpy-7p)]Cl-2, (bpy = 2,2'-bipyridine, m-bpy-7p = 4-methyl-4'-Arg-Gly-Asn-Ala-His-Glu-Arg-CONH2-2,2'-bipyridine) were synthesized and characterized and their binding properties to the deoxynucleotide duplexes d(5'-CGCGATCGCG-3')(2) and d(5'-GCGCTTAAGCGC-3')(2) were studied by means of H-1 NMR spectroscopy. 7p is part of the recognition loop of the restriction endonuclease MunI, a type II restriction enzyme from Mycoplasma unidentified which recognizes the palindromic hexanucleotide sequence C/AATTG and cleaves it as indicated by the slash. The A-isomer binds to the terminal CG/GC major groove of d(CGCGATCGCG)(2) decanucleotide, whereas the A-isomer approaches the GCT/CGA sequence. On the other hand, weak binding of the Delta-isomer to the end of d(GCGCTTAAGCGC)(2) into two different orientations is observed. In the case of the A-isomer, the bpy ligand(s) are located into the major groove of the central TT/AA sequence. The role of appended peptide sequences in sequence selectivity binding to DNA is being addressed. (C) 2004 Elsevier Inc. All rights reserved. (EN)

metallopeptides (EN)


J Inorg Biochem (EN)

English

2005


Elsevier (EN)



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