The position of the LysN(epsilon)H(2)-grafted antigens along the sequential oligopeptide carrier, Ac-(Aib-Lys-Aib-Gly)(n) (SOCn-II), influences the antibody recognition: Application to the Sm main autoimmune epitope
δείτε την πρωτότυπη σελίδα τεκμηρίου στον ιστότοπο του αποθετηρίου του φορέα για περισσότερες πληροφορίες και για να δείτε όλα τα ψηφιακά αρχεία του τεκμηρίου*
The position of the LysN(epsilon)H(2)-grafted antigens along the sequential oligopeptide carrier, Ac-(Aib-Lys-Aib-Gly)(n) (SOCn-II), influences the antibody recognition: Application to the Sm main autoimmune epitope
(EN)
Alexopoulos, C.
(EN)
Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας
(EL)
Alexopoulos, C.
(EN)
A sequential oligopeptide carrier of antigenic peptides is presented, incorporating two Aib residues in each repetitive moiety: Ac-(Aib-Lys-Aib-Gly)(n), (SOCn-II; n = 2-4). The conformational study, by H-1-nmr, CD, and Fourier transform ir spectroscopy; indicated that the SOCn-II carrier displays a pronounced 3(10)-helix, compared to the Ac-(Lys-Aib-Gly)(n) (SOCn-I) carrier of the same approximately backbone length, previously reported One of the dominant autoimmune epitopes of the Sm and U1RNP cellular components, the PPGMRPP sequence, was coupled to the Lys-(NH2)-H-epsilon groups of the SOCn-II carrier and used as antigenic substrate for detecting anti-Sm/ U1RNP autoantibodies in ELISA assays. Anti-Sm antibodies are highly specific for systemic lupus erythematosus, while anti-U1RNP are specific for mixed connective tissue disease. The anti-(PPGMRPP)(5)-SOCn-II ELISA was compared with the anti-(PPGMRPP)(n)-SOCn-I ELISA, provided that both antigenic substrates possess the same amount of the epitope replicates. The significance of the lysine positions along the oligopeptide backbone of the carrier for a favorable antibody recognition of the anchored antigens is also examined. (C) 2000 John Wiley & Sons, Inc. Biopoly 54: 1-10, 2000.
(EN)
*Η εύρυθμη και αδιάλειπτη λειτουργία των διαδικτυακών διευθύνσεων των συλλογών (ψηφιακό αρχείο, καρτέλα τεκμηρίου στο αποθετήριο) είναι αποκλειστική ευθύνη των αντίστοιχων Φορέων περιεχομένου.
Βοηθείστε μας να κάνουμε καλύτερο το OpenArchives.gr.