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L-lysine determination in animal feed using microbiological microtiter plate assay

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Προσδιορισμός L-λυσίνης σε ζωοτροφές χρησιμοποιώντας μικροβιολογικό πλάκας μικροτιτλοδότησηςπροσδιορισμό (EL)
L-lysine determination in animal feed using microbiological microtiter plate assay (EN)
JAJIĆ, I.
MIHALJEV, Ž.
KRSTOVIĆ, S.
JAKŠIĆ, S.
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
2018-10-10
ΔΕΝ ΔΙΑΤΙΘΕΤΑΙ ΠΕΡΙΛΗΨΗ ΣΤΑ ΕΛΛΗΝΙΚΑ (EL)
Chromatographic methods are most commonly used for the analysis of amino acids; however, there is growing need for faster, simpler and more price-effective assays. In this paper, the applicability of a rapid microbiological assay for quantification of the total content of L-lysine in feed samples was evaluated. The assay relies on the dependency of bacterial growth of Pediococcus acidilactici on the presence of L-lysine. Microbiological microtiter plate assay method for the quantitative determination of total (added and natural) L-lysine in feed samples has been verified, and parameters such as accuracy, precision, limit of detection, and limit of determination were evaluated. Results of total L-lysine determination in different feed samples have been compared with results of validated HPLC method. The microbiological microtiter plate assay method can be employed as a qualitative and quantification method for total L-lysine determination with detection and determination limit of 0.040 % and 0.085 %, respectively. However, further research on the influence of sample matrix on the determination of low lysine levels is required. (EN)
ζωοτροφές (EL)
μικροβιολογική τεχνική (EL)
L-λυσίνη (EL)
προσδιορισμός (EL)
HPLC (EL)
feed (EN)
L-lysine (EN)
determination (EN)
microbiological assay (EN)
HPLC (EN)
Journal of the Hellenic Veterinary Medical Society
English
Hellenic Veterinary Medical Society / Ελληνική Κτηνιατρική Εταιρεία (EN)
1792-2720
2585-3724
Περιοδικό της Ελληνικής Κτηνιατρικής Εταιρείας; Τόμ. 69 Αρ. 3 (2018); 1094-1100 (EL)
Journal of the Hellenic Veterinary Medical Society; Vol. 69 No. 3 (2018); 1094-1100 (EN)
Copyright (c) 2018 S. JAKŠIĆ, I. JAJIĆ, S. KRSTOVIĆ, Ž. MIHALJEV (EN)
https://creativecommons.org/licenses/by-nc/4.0
Hellenic Veterinary Medical Society
Journal of the Hellenic Veterinary Medical Society
Research Articles



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