Προσδιορισμός L-λυσίνης σε ζωοτροφές χρησιμοποιώντας μικροβιολογικό πλάκας μικροτιτλοδότησηςπροσδιορισμό
(EL)
L-lysine determination in animal feed using microbiological microtiter plate assay
(EN)
JAJIĆ, I.
MIHALJEV, Ž.
KRSTOVIĆ, S.
JAKŠIĆ, S.
ΔΕΝ ΔΙΑΤΙΘΕΤΑΙ ΠΕΡΙΛΗΨΗ ΣΤΑ ΕΛΛΗΝΙΚΑ
(EL)
Chromatographic methods are most commonly used for the analysis of amino acids; however, there is growing need for faster, simpler and more price-effective assays. In this paper, the applicability of a rapid microbiological assay for quantification of the total content of L-lysine in feed samples was evaluated. The assay relies on the dependency of bacterial growth of Pediococcus acidilactici on the presence of L-lysine. Microbiological microtiter plate assay method for the quantitative determination of total (added and natural) L-lysine in feed samples has been verified, and parameters such as accuracy, precision, limit of detection, and limit of determination were evaluated. Results of total L-lysine determination in different feed samples have been compared with results of validated HPLC method. The microbiological microtiter plate assay method can be employed as a qualitative and quantification method for total L-lysine determination with detection and determination limit of 0.040 % and 0.085 %, respectively. However, further research on the influence of sample matrix on the determination of low lysine levels is required.
(EN)
