Absorption and fluorescence spectroscopy of biological tissues,by using intense pulsed laser ,in order to detect differences between pathological and healthy tissues
Φασματοσκοπία απορρόφησης και εκπομπής βιολογικών δειγμάτων με χρήση έντονων παλμών laser ,με σκοπό τη διαφοροποίηση παθολογικών απο υγιείς ιστούς
Τσιλιμπάρης , Μιλτιάδης
The aim of this project is to measure the absorption and fluorescence spectroscopy of biological tissues in order to detect differences between pathological and healthy tissues. In order to achieve our goal we used special high technological machines which were provided to us by ITE. As far as the absorption spectroscopy is concerned we used perkin elmer lambda 950 spectrometer which is able to measure a wide range of spectre from 2500 nm to 25nm. As far as the fluorescence spectroscopy is concerned we carried out our measurements thanks to a powerful Laser that ITE posseses, the femtopower compact pro Laser.
Diagnostic techniques based on absorption and fluorescence spectroscopy have the potential to link the biochemical and morphologic properties of tissues to individual patient care. In particular, these techniques are fast, non-invasive and quantitative. Furthermore, they can be used to elucidate key tissue features, such as the cellular metabolic rate, vascularity, intra-vascular oxygenation and alterations in tissue morphology. These tissue features can be interpreted to shed light on a variety of clinical problems, such as neoplasia and atherosclerosis. If applied successfully, absorption and fluorescence spectroscopy has the potential to represent an important step forward toward advances in diagnostic and therapeutic medical applications